On the site specific recombination of phage 16-3 of Rhizobium meliloti: identification of genetic elements and att recombinations

F. Olasz, László Dorgai, Péter Papp, E. Hermesz, Edit Kósa, L. Orosz

Research output: Article

9 Citations (Scopus)

Abstract

The genome segment carrying the activities int and xis, responsible for integration and excision of phage 16-3, have been identified and cloned. Mutants were isolated, permitting the investigation of int, xis and att sites (attP, attR, attB) in trans arrangements. The efficiency and role of int- and xis-promoted reactions and of homologous recombination in the formation of lysogenic cells are established. The possible use of the cloned int-attP chromosomal segment in the manipulation of Rhizobium meliloti is discussed.

Original languageEnglish
Pages (from-to)289-295
Number of pages7
JournalMGG Molecular & General Genetics
Volume201
Issue number2
DOIs
Publication statusPublished - nov. 1985

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Sinorhizobium meliloti
Homologous Recombination
Bacteriophages
Genetic Recombination
Genome

ASJC Scopus subject areas

  • Genetics

Cite this

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abstract = "The genome segment carrying the activities int and xis, responsible for integration and excision of phage 16-3, have been identified and cloned. Mutants were isolated, permitting the investigation of int, xis and att sites (attP, attR, attB) in trans arrangements. The efficiency and role of int- and xis-promoted reactions and of homologous recombination in the formation of lysogenic cells are established. The possible use of the cloned int-attP chromosomal segment in the manipulation of Rhizobium meliloti is discussed.",
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T1 - On the site specific recombination of phage 16-3 of Rhizobium meliloti

T2 - identification of genetic elements and att recombinations

AU - Olasz, F.

AU - Dorgai, László

AU - Papp, Péter

AU - Hermesz, E.

AU - Kósa, Edit

AU - Orosz, L.

PY - 1985/11

Y1 - 1985/11

N2 - The genome segment carrying the activities int and xis, responsible for integration and excision of phage 16-3, have been identified and cloned. Mutants were isolated, permitting the investigation of int, xis and att sites (attP, attR, attB) in trans arrangements. The efficiency and role of int- and xis-promoted reactions and of homologous recombination in the formation of lysogenic cells are established. The possible use of the cloned int-attP chromosomal segment in the manipulation of Rhizobium meliloti is discussed.

AB - The genome segment carrying the activities int and xis, responsible for integration and excision of phage 16-3, have been identified and cloned. Mutants were isolated, permitting the investigation of int, xis and att sites (attP, attR, attB) in trans arrangements. The efficiency and role of int- and xis-promoted reactions and of homologous recombination in the formation of lysogenic cells are established. The possible use of the cloned int-attP chromosomal segment in the manipulation of Rhizobium meliloti is discussed.

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