The glow band appearing at around +50°C was studied in various subchloroplast preparations. The band was intensified by addition of detergent to the chloroplast suspension and it was present in all preparations obtained by detergent treatment as well as inartificially prepared protein-pigment-detergent micelles. The band could only be generated by continuous light, and not with flash excitation, and it disappeared when the detergent concentration was decreased in the sample. Our results indicate that the band appearing in subchloroplast preparations at around + 50°C is an artifact generated by detergent-protein-pigment interaction and it has to be distinguished from the + 50°C band observable in leaves and isolated chloroplasts.
ASJC Scopus subject areas
- Cell Biology