On the determination of the helical structure parameters of amyloid protofilaments by small-angle neutron scattering and atomic force microscopy

Mikhail V. Avdeev, V. Aksenov, Zuzana Gazová, L. Almásy, Viktor I. Petrenko, Hubert Gojzewski, Artem V. Feoktystov, Katarina Siposova, Andrea Antosova, Milan Timko, Peter Kopcansky

Research output: Article

9 Citations (Scopus)

Abstract

The helical structure of amyloid protofilaments of hen egg white lysozyme was analyzed by small-angle neutron scattering (SANS) and atomic force microscopy (AFM). The structure of these formations in bulk solutions was adequately described by SANS in terms of a simplified model of a helix with spherical structural units. The found main helix parameters (pitch and effective diameter) are consistent with the results of AFM analysis for amyloid fibrils adsorbed on a mica surface. Both methods reveal a strong isotope effect on the structure of amyloid fibrils with respect to the substitution of heavy for light water in the solvent. Specific details responsible for the structural differences when comparing SANS and AFM data are discussed from the viewpoint of methodological aspects, the influence of different (native and adsorbed) amyloid states and sample preparation.

Original languageEnglish
Pages (from-to)224-233
Number of pages10
JournalJournal of Applied Crystallography
Volume46
Issue number1
DOIs
Publication statusPublished - febr. 2013

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Small Angle Scattering
Atomic Force Microscopy
Neutrons
Neutron scattering
Amyloid
Atomic force microscopy
Deuterium Oxide
Egg White
Muramidase
Isotopes
Substitution reactions
Water

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

On the determination of the helical structure parameters of amyloid protofilaments by small-angle neutron scattering and atomic force microscopy. / Avdeev, Mikhail V.; Aksenov, V.; Gazová, Zuzana; Almásy, L.; Petrenko, Viktor I.; Gojzewski, Hubert; Feoktystov, Artem V.; Siposova, Katarina; Antosova, Andrea; Timko, Milan; Kopcansky, Peter.

In: Journal of Applied Crystallography, Vol. 46, No. 1, 02.2013, p. 224-233.

Research output: Article

Avdeev, Mikhail V. ; Aksenov, V. ; Gazová, Zuzana ; Almásy, L. ; Petrenko, Viktor I. ; Gojzewski, Hubert ; Feoktystov, Artem V. ; Siposova, Katarina ; Antosova, Andrea ; Timko, Milan ; Kopcansky, Peter. / On the determination of the helical structure parameters of amyloid protofilaments by small-angle neutron scattering and atomic force microscopy. In: Journal of Applied Crystallography. 2013 ; Vol. 46, No. 1. pp. 224-233.
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AU - Avdeev, Mikhail V.

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AU - Gazová, Zuzana

AU - Almásy, L.

AU - Petrenko, Viktor I.

AU - Gojzewski, Hubert

AU - Feoktystov, Artem V.

AU - Siposova, Katarina

AU - Antosova, Andrea

AU - Timko, Milan

AU - Kopcansky, Peter

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