Abstract
Two dUTPase isoforms (23 kDa and 21 kDa) are present in the fruitfly with the sole difference of an N-terminal extension. In Drosophila embryo, both isoforms are detected inside the nucleus. Here, we investigated the function of the N-terminal segment using eYFP-dUTPase constructs. In Schneider 2 cells, only the 23 kDa construct showed nuclear localization arguing that it may contain a nuclear localization signal (NLS). Sequence comparisons identified a lysine-rich nonapeptide with similarity to the human c-myc NLS. In Drosophila embryos during nuclear cleavages, the 23 kDa isoform showed the expected localization shifts. Contrariwise, although the 21 kDa isoform was excluded from the nuclei during interphase, it was shifted to the nucleus during prophase and forthcoming mitotic steps. The observed dynamic localization character showed strict timing to the nuclear cleavage phases and explained how both isoforms can be present within the nuclear microenvironment, although at different stages of cell cycle.
| Original language | English |
|---|---|
| Pages (from-to) | 271-275 |
| Number of pages | 5 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 381 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - ápr. 3 2009 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Cell Biology
- Molecular Biology
Cite this
Nuclear localization signal-dependent and -independent movements of Drosophila melanogaster dUTPase isoforms during nuclear cleavage. / Muha, Villo; Zagyva, Imre; Venkei, Zsolt; Szabad, J.; Vértessy, B.
In: Biochemical and Biophysical Research Communications, Vol. 381, No. 2, 03.04.2009, p. 271-275.Research output: Article
}
TY - JOUR
T1 - Nuclear localization signal-dependent and -independent movements of Drosophila melanogaster dUTPase isoforms during nuclear cleavage
AU - Muha, Villo
AU - Zagyva, Imre
AU - Venkei, Zsolt
AU - Szabad, J.
AU - Vértessy, B.
PY - 2009/4/3
Y1 - 2009/4/3
N2 - Two dUTPase isoforms (23 kDa and 21 kDa) are present in the fruitfly with the sole difference of an N-terminal extension. In Drosophila embryo, both isoforms are detected inside the nucleus. Here, we investigated the function of the N-terminal segment using eYFP-dUTPase constructs. In Schneider 2 cells, only the 23 kDa construct showed nuclear localization arguing that it may contain a nuclear localization signal (NLS). Sequence comparisons identified a lysine-rich nonapeptide with similarity to the human c-myc NLS. In Drosophila embryos during nuclear cleavages, the 23 kDa isoform showed the expected localization shifts. Contrariwise, although the 21 kDa isoform was excluded from the nuclei during interphase, it was shifted to the nucleus during prophase and forthcoming mitotic steps. The observed dynamic localization character showed strict timing to the nuclear cleavage phases and explained how both isoforms can be present within the nuclear microenvironment, although at different stages of cell cycle.
AB - Two dUTPase isoforms (23 kDa and 21 kDa) are present in the fruitfly with the sole difference of an N-terminal extension. In Drosophila embryo, both isoforms are detected inside the nucleus. Here, we investigated the function of the N-terminal segment using eYFP-dUTPase constructs. In Schneider 2 cells, only the 23 kDa construct showed nuclear localization arguing that it may contain a nuclear localization signal (NLS). Sequence comparisons identified a lysine-rich nonapeptide with similarity to the human c-myc NLS. In Drosophila embryos during nuclear cleavages, the 23 kDa isoform showed the expected localization shifts. Contrariwise, although the 21 kDa isoform was excluded from the nuclei during interphase, it was shifted to the nucleus during prophase and forthcoming mitotic steps. The observed dynamic localization character showed strict timing to the nuclear cleavage phases and explained how both isoforms can be present within the nuclear microenvironment, although at different stages of cell cycle.
KW - dUTPase
KW - Nuclear cleavage
KW - Nuclear localization signal
KW - Nucleo-cytoplasmic transport
UR - http://www.scopus.com/inward/record.url?scp=61849114645&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=61849114645&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2009.02.036
DO - 10.1016/j.bbrc.2009.02.036
M3 - Article
C2 - 19232319
AN - SCOPUS:61849114645
VL - 381
SP - 271
EP - 275
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -