Novel developments in rapid mycotoxin detection

S. De Saeger, L. Sibanda, C. Paepens, M. Lobeau, B. Delmulle, I. Barna-Vetró, C. Van Peteghem

Research output: Article

10 Citations (Scopus)

Abstract

Rapid antibody-based mycotoxin screening techniques are designed to be used outside a laboratory environment, at the place of sampling. Results are expected immediately, so that commodities can be further processed without delay. Because they are used for mycotoxin analysis, very low levels (ppb and ppt range) should be detected. A further requirement is that the obtained results are accurate with a false negative rate of 1 in pig feed a cut-off value of 5 μg/kg could be reached with no false negative results and a false positive rate of only 10%. Sample pretreatment for screening techniques should be rapid and simple. Preferably a simple solvent extraction is used, followed by a filtration and dilution step. However, for strongly coloured or complex food matrices, this did not seem to work. The combination of clean-up and detection in one single test device is a new approach. When using this clean-up tandem assay column for the detection of ochratoxin A in roasted coffee, a cut-off value of 6 μg/kg was reached. No false positive results were obtained, however, the false negative rate was 8%.

Original languageEnglish
Pages (from-to)100-104
Number of pages5
JournalMycotoxin Research
Volume22
Issue number2
DOIs
Publication statusPublished - jún. 2006

Fingerprint

Mycotoxins
Screening
Coffee
Solvent extraction
Dilution
Assays
Swine
Sampling
Food
Equipment and Supplies
Antibodies
ochratoxin A

ASJC Scopus subject areas

  • Microbiology
  • Toxicology
  • Biotechnology

Cite this

De Saeger, S., Sibanda, L., Paepens, C., Lobeau, M., Delmulle, B., Barna-Vetró, I., & Van Peteghem, C. (2006). Novel developments in rapid mycotoxin detection. Mycotoxin Research, 22(2), 100-104. https://doi.org/10.1007/BF02956772

Novel developments in rapid mycotoxin detection. / De Saeger, S.; Sibanda, L.; Paepens, C.; Lobeau, M.; Delmulle, B.; Barna-Vetró, I.; Van Peteghem, C.

In: Mycotoxin Research, Vol. 22, No. 2, 06.2006, p. 100-104.

Research output: Article

De Saeger, S, Sibanda, L, Paepens, C, Lobeau, M, Delmulle, B, Barna-Vetró, I & Van Peteghem, C 2006, 'Novel developments in rapid mycotoxin detection', Mycotoxin Research, vol. 22, no. 2, pp. 100-104. https://doi.org/10.1007/BF02956772
De Saeger S, Sibanda L, Paepens C, Lobeau M, Delmulle B, Barna-Vetró I et al. Novel developments in rapid mycotoxin detection. Mycotoxin Research. 2006 jún.;22(2):100-104. https://doi.org/10.1007/BF02956772
De Saeger, S. ; Sibanda, L. ; Paepens, C. ; Lobeau, M. ; Delmulle, B. ; Barna-Vetró, I. ; Van Peteghem, C. / Novel developments in rapid mycotoxin detection. In: Mycotoxin Research. 2006 ; Vol. 22, No. 2. pp. 100-104.
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