Novel Amperometric Tri-Enzyme Biosensor for Lysine Determination in Pharmaceutical Products and Food Samples

Beáta Bóka, Marietta Korózs, Márta Nánási, N. Adányi

Research output: Article

7 Citations (Scopus)

Abstract

An enzyme-based amperometric biosensor was developed for the determination of the lysine content of pharmaceutical products, food and feed samples. Lysine decarboxylase (EC 4.1.1.18) from Bacterium cadaveris was coupled with diamine oxidase (EC 1.4.3.6) from Pisum sativum and horseradish peroxidase (EC 1.11.1.7). The enzymes were co-immobilized on a graphite electrode with an Os polymer. The biosensor worked in flow injection analysis system (FIA). Optimal working conditions were pH7.0 and -50mV potential vs. Ag/AgCl. The linear measuring range was 0.005-0.500mM. Different pharmaceutical products and feed samples were analysed by the lysine biosensor and by HPLC method for validation.

Original languageEnglish
Pages (from-to)817-824
Number of pages8
JournalElectroanalysis
Volume27
Issue number3
DOIs
Publication statusPublished - márc. 1 2015

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Biosensors
Drug products
Lysine
Enzymes
lysine decarboxylase
Pharmaceutical Preparations
Amine Oxidase (Copper-Containing)
Graphite electrodes
Horseradish Peroxidase
Bacteria
Polymers

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

Cite this

Novel Amperometric Tri-Enzyme Biosensor for Lysine Determination in Pharmaceutical Products and Food Samples. / Bóka, Beáta; Korózs, Marietta; Nánási, Márta; Adányi, N.

In: Electroanalysis, Vol. 27, No. 3, 01.03.2015, p. 817-824.

Research output: Article

Bóka, Beáta ; Korózs, Marietta ; Nánási, Márta ; Adányi, N. / Novel Amperometric Tri-Enzyme Biosensor for Lysine Determination in Pharmaceutical Products and Food Samples. In: Electroanalysis. 2015 ; Vol. 27, No. 3. pp. 817-824.
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