Nodulation signaling in legumes requires NSP2, a member of the GRAS family of transcriptional regulators

P. Kaló, Cynthia Gleason, Anne Edwards, John Marsh, Raka M. Mitra, Sibylle Hirsch, Júlia Jakab, Sarah Sims, Sharon R. Long, Jane Rogers, G. Kiss, J. Allan Downie, Giles E D Oldroyd

Research output: Article

354 Citations (Scopus)

Abstract

Rhizobial bacteria enter a symbiotic interaction with legumes, activating diverse responses in roots through the lipochito oligosaccharide signaling molecule Nod factor. Here, we show that NSP2 from Medicago truncatula encodes a GRAS protein essential for Nod-factor signaling. NSP2 functions downstream of Nod-factor-induced calcium spiking and a calcium/calmodulin-dependent protein kinase. We show that NSP2-GFP expressed from a constitutive promoter is localized to the endoplasmic reticulum/nuclear envelope and relocalizes to the nucleus after Nod-factor elicitation. This work provides evidence that a GRAS protein transduces calcium signals in plants and provides a possible regulator of Nod-factor-inducible gene expression.

Original languageEnglish
Pages (from-to)1786-1789
Number of pages4
JournalScience
Volume308
Issue number5729
DOIs
Publication statusPublished - jún. 17 2005

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Fabaceae
Medicago truncatula
Calcium
Calcium-Calmodulin-Dependent Protein Kinases
Nuclear Envelope
Oligosaccharides
Endoplasmic Reticulum
Proteins
Bacteria
Gene Expression

ASJC Scopus subject areas

  • General

Cite this

Kaló, P., Gleason, C., Edwards, A., Marsh, J., Mitra, R. M., Hirsch, S., ... Oldroyd, G. E. D. (2005). Nodulation signaling in legumes requires NSP2, a member of the GRAS family of transcriptional regulators. Science, 308(5729), 1786-1789. https://doi.org/10.1126/science.1110951

Nodulation signaling in legumes requires NSP2, a member of the GRAS family of transcriptional regulators. / Kaló, P.; Gleason, Cynthia; Edwards, Anne; Marsh, John; Mitra, Raka M.; Hirsch, Sibylle; Jakab, Júlia; Sims, Sarah; Long, Sharon R.; Rogers, Jane; Kiss, G.; Downie, J. Allan; Oldroyd, Giles E D.

In: Science, Vol. 308, No. 5729, 17.06.2005, p. 1786-1789.

Research output: Article

Kaló, P, Gleason, C, Edwards, A, Marsh, J, Mitra, RM, Hirsch, S, Jakab, J, Sims, S, Long, SR, Rogers, J, Kiss, G, Downie, JA & Oldroyd, GED 2005, 'Nodulation signaling in legumes requires NSP2, a member of the GRAS family of transcriptional regulators', Science, vol. 308, no. 5729, pp. 1786-1789. https://doi.org/10.1126/science.1110951
Kaló, P. ; Gleason, Cynthia ; Edwards, Anne ; Marsh, John ; Mitra, Raka M. ; Hirsch, Sibylle ; Jakab, Júlia ; Sims, Sarah ; Long, Sharon R. ; Rogers, Jane ; Kiss, G. ; Downie, J. Allan ; Oldroyd, Giles E D. / Nodulation signaling in legumes requires NSP2, a member of the GRAS family of transcriptional regulators. In: Science. 2005 ; Vol. 308, No. 5729. pp. 1786-1789.
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