No evidence for disturbed COL1A1 and A2 expression in otosclerosis

Péter Csomor, Balázs Liktor, Bálint Liktor, István Sziklai, Tamás Karosi

Research output: Article

6 Citations (Scopus)

Abstract

Otosclerosis is a complex bone remodeling disorder of the human otic capsule that might be associated with various mutations of A1 and A2 alleles of type-I collagen. The study herein presented, investigates the possibilty of the genetic involvement of type-I collagen in the pathogenesis of histologically conWrmed otosclerosis. A total of 55 ankylotic stapes footplates were analyzed. Cortical bone fragments (n = 30), incus (n = 3) and malleus (n = 2) specimens were employed as negative controls. Specimens were divided into two groups. The Wrst group was processed using conventional H.E. hematoxylin-eosin (H.E.) staining and type-I collagen-speciWc immunoXuorescent assay (IFA), while the second group was examined by COL1A1 and A2-speciWc RT-PCR. Otosclerotic- (n = 31) and nonotosclerotic stapes footplates (n = 9) as well as cortical bones (n = 20), incus (n = 2) and malleus specimens (n = 1) showed normal and quite similar A1 and A2 allele expression conWrmed by IFA. RT-PCR analysis revealed normal and consistent mRNA expression of both alleles in each specimen. Expression levels and patterns of COL1A1/A2 alleles did not show signiWcant correlation with the histological diagnosis of otosclerosis. Type-I collagen is a highly conserved structure protein, which plays a fundamental role in the integritiy of various connective tissues. Mutations of A1 and A2 alleles result in serious systemic disorders of the skeleton, tendons and skin. Since otosclerosis is an organ-speciWc disease, it is diYcult to explain its genetic association with type-I collagen. In conclusion, we found no evidence supporting the putative link of COL1A1 and COL1A2 alleles with otosclerosis.

Original languageEnglish
Pages (from-to)2043-2051
Number of pages9
JournalEuropean Archives of Oto-Rhino-Laryngology
Volume269
Issue number9
DOIs
Publication statusPublished - szept. 1 2012

ASJC Scopus subject areas

  • Otorhinolaryngology

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