NMR metabolomics defining genetic variation in pea seed metabolites

Noel Ellis, Chie Hattori, Jitender Cheema, James Donarski, Adrian Charlton, Michael Dickinson, Giampaolo Venditti, P. Kaló, Zoltán Szabó, G. Kiss, Claire Domoney

Research output: Article

4 Citations (Scopus)

Abstract

Nuclear magnetic resonance (NMR) spectroscopy profiling was used to provide an unbiased assessment of changes to the metabolite composition of seeds and to define genetic variation for a range of pea seed metabolites. Mature seeds from recombinant inbredlines, derivedfromthree mapping populations for which there is substantial genetic marker linkage information, were grown in two environments/years and analyzed by non-targeted NMR. Adaptive binning of the NMRmetabolite data, followed by analysis of quantitative variation among lines for individual bins, identified the main genomic regions determining this metabolic variability and the variability for selected compounds was investigated. Analysis by t-tests identified a set of bins with highly significant associations to genetic map regions, based on probability (p) values that were appreciably lower than those determined for randomized data. The correlation between bins showing high mean absolute deviation and those showing low p-values for marker association provided an indication of the extent to which the genetics of bin variation might be explained by one or a few loci. Variation in compounds related to aromatic amino acids, branched-chain amino acids, sucrose-derived metabolites, secondary metabolites and some unidentified compounds was associated with one or more genetic loci. The combined analysis shows that there are multiple loci throughout the genome that together impact on the abundance of many compounds through a network of interactions, where individual loci may affect more than one compound and vice versa. This work therefore provides a framework for the genetic analysis of the seed metabolome, and the use of genetic marker data in the breeding and selection of seeds for specific seed quality traits and compounds that have high commercial value.

Original languageEnglish
Article number1022
JournalFrontiers in Plant Science
Volume9
DOIs
Publication statusPublished - júl. 17 2018

Fingerprint

metabolomics
nuclear magnetic resonance spectroscopy
peas
metabolites
genetic variation
seeds
loci
metabolome
genetic markers
branched chain amino acids
seed quality
secondary metabolites
linkage (genetics)
aromatic compounds
genetic techniques and protocols
quantitative analysis
sucrose
genomics
amino acids
genome

ASJC Scopus subject areas

  • Plant Science

Cite this

Ellis, N., Hattori, C., Cheema, J., Donarski, J., Charlton, A., Dickinson, M., ... Domoney, C. (2018). NMR metabolomics defining genetic variation in pea seed metabolites. Frontiers in Plant Science, 9, [1022]. https://doi.org/10.3389/fpls.2018.01022

NMR metabolomics defining genetic variation in pea seed metabolites. / Ellis, Noel; Hattori, Chie; Cheema, Jitender; Donarski, James; Charlton, Adrian; Dickinson, Michael; Venditti, Giampaolo; Kaló, P.; Szabó, Zoltán; Kiss, G.; Domoney, Claire.

In: Frontiers in Plant Science, Vol. 9, 1022, 17.07.2018.

Research output: Article

Ellis, N, Hattori, C, Cheema, J, Donarski, J, Charlton, A, Dickinson, M, Venditti, G, Kaló, P, Szabó, Z, Kiss, G & Domoney, C 2018, 'NMR metabolomics defining genetic variation in pea seed metabolites', Frontiers in Plant Science, vol. 9, 1022. https://doi.org/10.3389/fpls.2018.01022
Ellis N, Hattori C, Cheema J, Donarski J, Charlton A, Dickinson M et al. NMR metabolomics defining genetic variation in pea seed metabolites. Frontiers in Plant Science. 2018 júl. 17;9. 1022. https://doi.org/10.3389/fpls.2018.01022
Ellis, Noel ; Hattori, Chie ; Cheema, Jitender ; Donarski, James ; Charlton, Adrian ; Dickinson, Michael ; Venditti, Giampaolo ; Kaló, P. ; Szabó, Zoltán ; Kiss, G. ; Domoney, Claire. / NMR metabolomics defining genetic variation in pea seed metabolites. In: Frontiers in Plant Science. 2018 ; Vol. 9.
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