Nitric oxide–cytokinin interplay influences selenite sensitivity in Arabidopsis

Nóra Lehotai, Gábor Feigl, Ágnes Koós, Árpád Molnár, Attila Ördög, Andrea Pető, László Erdei, Zsuzsanna Kolbert

Research output: Article

11 Citations (Scopus)

Abstract

Key message: Selenite oppositely modifies cytokinin and nitric oxide metabolism inArabidopsisorgans. A mutually negative interplay between the molecules exists in selenite-exposed roots; and their overproduction causes selenite insensitivity.Abstract: Selenium-induced phytotoxicity is accompanied by developmental alterations such as primary root (PR) shortening. Growth changes are provoked by the modulation of hormone status and signalling. Cytokinin (CK) cooperates with the nitric oxide (NO) in many aspects of plant development; however, their interaction under abiotic stress has not been examined. Selenite inhibited the growth of Arabidopsis seedlings and reduced root meristem size through cell division arrest. The CK-dependent pARR5::GUS activity revealed the intensification of CK signalling in the PR tip, which may be partly responsible for the root meristem shortening. The selenite-induced alterations in the in situ expressions of cytokinin oxidases (AtCKX4::GUS, AtCKX5::GUS) are associated with selenite-triggered changes of CK signalling. In wild-type (WT) and NO-deficient nia1nia2 root, selenite led to the diminution of NO content, but CK overproducer ipt-161 and -deficient 35S:CKX2 roots did not show NO decrease. Exogenous NO (S-nitroso-N-acetyl-DL-penicillamine, SNAP) reduced the pARR5::GFP and pTCS::GFP expressions. Roots of the 35S:CKX and cyr1 plants suffered more severe selenite-triggered viability loss than the WT, while in ipt-161 and gsnor1-3 no obvious viability decrease was observed. Exogenous NO ameliorated viability loss, but benzyladenine intensified it. Based on the results, selenite impacts development by oppositely modifying CK signalling and NO level. In the root system, CK signalling intensifies which possibly contributes to the nitrate reductase-independent NO diminution. A mutually negative CK-NO interplay exists in selenite-exposed roots; however, overproduction of both molecules worsens selenite sensing. Hereby, we suggest novel regulatory interplay and role for NO and CK in abiotic stress signalling.

Original languageEnglish
Pages (from-to)1-15
Number of pages15
JournalPlant Cell Reports
DOIs
Publication statusAccepted/In press - júl. 23 2016

Fingerprint

selenites
cytokinins
nitric oxide
Arabidopsis
root meristems
viability
shortenings
abiotic stress
penicillamine
phytotoxicity
nitrate reductase
root tips
benzyladenine
selenium
root systems
plant development
cell division
hormones

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Lehotai, N., Feigl, G., Koós, Á., Molnár, Á., Ördög, A., Pető, A., ... Kolbert, Z. (Accepted/In press). Nitric oxide–cytokinin interplay influences selenite sensitivity in Arabidopsis. Plant Cell Reports, 1-15. https://doi.org/10.1007/s00299-016-2028-5

Nitric oxide–cytokinin interplay influences selenite sensitivity in Arabidopsis. / Lehotai, Nóra; Feigl, Gábor; Koós, Ágnes; Molnár, Árpád; Ördög, Attila; Pető, Andrea; Erdei, László; Kolbert, Zsuzsanna.

In: Plant Cell Reports, 23.07.2016, p. 1-15.

Research output: Article

Lehotai, N, Feigl, G, Koós, Á, Molnár, Á, Ördög, A, Pető, A, Erdei, L & Kolbert, Z 2016, 'Nitric oxide–cytokinin interplay influences selenite sensitivity in Arabidopsis', Plant Cell Reports, pp. 1-15. https://doi.org/10.1007/s00299-016-2028-5
Lehotai, Nóra ; Feigl, Gábor ; Koós, Ágnes ; Molnár, Árpád ; Ördög, Attila ; Pető, Andrea ; Erdei, László ; Kolbert, Zsuzsanna. / Nitric oxide–cytokinin interplay influences selenite sensitivity in Arabidopsis. In: Plant Cell Reports. 2016 ; pp. 1-15.
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AU - Kolbert, Zsuzsanna

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N2 - Key message: Selenite oppositely modifies cytokinin and nitric oxide metabolism inArabidopsisorgans. A mutually negative interplay between the molecules exists in selenite-exposed roots; and their overproduction causes selenite insensitivity.Abstract: Selenium-induced phytotoxicity is accompanied by developmental alterations such as primary root (PR) shortening. Growth changes are provoked by the modulation of hormone status and signalling. Cytokinin (CK) cooperates with the nitric oxide (NO) in many aspects of plant development; however, their interaction under abiotic stress has not been examined. Selenite inhibited the growth of Arabidopsis seedlings and reduced root meristem size through cell division arrest. The CK-dependent pARR5::GUS activity revealed the intensification of CK signalling in the PR tip, which may be partly responsible for the root meristem shortening. The selenite-induced alterations in the in situ expressions of cytokinin oxidases (AtCKX4::GUS, AtCKX5::GUS) are associated with selenite-triggered changes of CK signalling. In wild-type (WT) and NO-deficient nia1nia2 root, selenite led to the diminution of NO content, but CK overproducer ipt-161 and -deficient 35S:CKX2 roots did not show NO decrease. Exogenous NO (S-nitroso-N-acetyl-DL-penicillamine, SNAP) reduced the pARR5::GFP and pTCS::GFP expressions. Roots of the 35S:CKX and cyr1 plants suffered more severe selenite-triggered viability loss than the WT, while in ipt-161 and gsnor1-3 no obvious viability decrease was observed. Exogenous NO ameliorated viability loss, but benzyladenine intensified it. Based on the results, selenite impacts development by oppositely modifying CK signalling and NO level. In the root system, CK signalling intensifies which possibly contributes to the nitrate reductase-independent NO diminution. A mutually negative CK-NO interplay exists in selenite-exposed roots; however, overproduction of both molecules worsens selenite sensing. Hereby, we suggest novel regulatory interplay and role for NO and CK in abiotic stress signalling.

AB - Key message: Selenite oppositely modifies cytokinin and nitric oxide metabolism inArabidopsisorgans. A mutually negative interplay between the molecules exists in selenite-exposed roots; and their overproduction causes selenite insensitivity.Abstract: Selenium-induced phytotoxicity is accompanied by developmental alterations such as primary root (PR) shortening. Growth changes are provoked by the modulation of hormone status and signalling. Cytokinin (CK) cooperates with the nitric oxide (NO) in many aspects of plant development; however, their interaction under abiotic stress has not been examined. Selenite inhibited the growth of Arabidopsis seedlings and reduced root meristem size through cell division arrest. The CK-dependent pARR5::GUS activity revealed the intensification of CK signalling in the PR tip, which may be partly responsible for the root meristem shortening. The selenite-induced alterations in the in situ expressions of cytokinin oxidases (AtCKX4::GUS, AtCKX5::GUS) are associated with selenite-triggered changes of CK signalling. In wild-type (WT) and NO-deficient nia1nia2 root, selenite led to the diminution of NO content, but CK overproducer ipt-161 and -deficient 35S:CKX2 roots did not show NO decrease. Exogenous NO (S-nitroso-N-acetyl-DL-penicillamine, SNAP) reduced the pARR5::GFP and pTCS::GFP expressions. Roots of the 35S:CKX and cyr1 plants suffered more severe selenite-triggered viability loss than the WT, while in ipt-161 and gsnor1-3 no obvious viability decrease was observed. Exogenous NO ameliorated viability loss, but benzyladenine intensified it. Based on the results, selenite impacts development by oppositely modifying CK signalling and NO level. In the root system, CK signalling intensifies which possibly contributes to the nitrate reductase-independent NO diminution. A mutually negative CK-NO interplay exists in selenite-exposed roots; however, overproduction of both molecules worsens selenite sensing. Hereby, we suggest novel regulatory interplay and role for NO and CK in abiotic stress signalling.

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