Nigella sativa essential oil and its bioactive compounds as resistance modifiers against Staphylococcus aureus

Ahmad Mouwakeh, Annamária Kincses, Márta Nové, Tímea Mosolygó, Csilla Mohácsi-Farkas, Gabriella Kiskó, G. Spengler

Research output: Article

Abstract

Nigella sativa essential oil (EO) and its compounds (thymoquinone, carvacrol, and p-cymene) have a broad antimicrobial spectrum. The aim of this study was to investigate the antimicrobial and resistance modifying activity of N. sativa EO, thymoquinone, carvacrol, and p-cymene against one methicillin susceptible and one methicillin resistant Staphylococcus aureus strain. N. sativa EO, thymoquinone, carvacrol, and p-cymene were assessed for antimicrobial activity and modulation of antimicrobial resistance (by broth microdilution), inhibition of antimicrobial efflux (by ethidium bromide [EtBr] accumulation assay), relative expression of mepA gene (by real-time reverse transcriptase quantitative polymerase chain reaction), membrane disrupting effect (by LIVE/DEAD BacLight™ Kit), and finally antibiofilm activity (by the crystal violet assay). Both strains of S. aureus were susceptible to N. sativa EO, thymoquinone, and carvacrol. N. sativa EO and carvacrol induced the increase of EtBr accumulated by both S. aureus strains. Membrane integrity of ATCC strain was disrupted by carvacrol and p-cymene, whereas for the methicillin resistant S. aureus (MRSA) strain the membrane integrity was disrupted by each compound. N. sativa EO and its bioactive compounds such as carvacrol and p-cymene could be applied as resistance modifiers in MRSA strains.

Original languageEnglish
JournalPhytotherapy Research
DOIs
Publication statusAccepted/In press - jan. 1 2019

Fingerprint

Volatile Oils
Staphylococcus aureus
Methicillin Resistance
Ethidium
Membranes
Gentian Violet
Methicillin
Methicillin-Resistant Staphylococcus aureus
caraway oil
carvacrol
Reverse Transcriptase Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
4-cymene
Gene Expression
thymoquinone

ASJC Scopus subject areas

  • Pharmacology

Cite this

Nigella sativa essential oil and its bioactive compounds as resistance modifiers against Staphylococcus aureus. / Mouwakeh, Ahmad; Kincses, Annamária; Nové, Márta; Mosolygó, Tímea; Mohácsi-Farkas, Csilla; Kiskó, Gabriella; Spengler, G.

In: Phytotherapy Research, 01.01.2019.

Research output: Article

Mouwakeh, Ahmad ; Kincses, Annamária ; Nové, Márta ; Mosolygó, Tímea ; Mohácsi-Farkas, Csilla ; Kiskó, Gabriella ; Spengler, G. / Nigella sativa essential oil and its bioactive compounds as resistance modifiers against Staphylococcus aureus. In: Phytotherapy Research. 2019.
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