Nigella sativa essential oil and its bioactive compounds as resistance modifiers against Staphylococcus aureus

Ahmad Mouwakeh, Annamária Kincses, Márta Nové, Tímea Mosolygó, Csilla Mohácsi-Farkas, Gabriella Kiskó, G. Spengler

Research output: Article

Abstract

Nigella sativa essential oil (EO) and its compounds (thymoquinone, carvacrol, and p-cymene) have a broad antimicrobial spectrum. The aim of this study was to investigate the antimicrobial and resistance modifying activity of N. sativa EO, thymoquinone, carvacrol, and p-cymene against one methicillin susceptible and one methicillin resistant Staphylococcus aureus strain. N. sativa EO, thymoquinone, carvacrol, and p-cymene were assessed for antimicrobial activity and modulation of antimicrobial resistance (by broth microdilution), inhibition of antimicrobial efflux (by ethidium bromide [EtBr] accumulation assay), relative expression of mepA gene (by real-time reverse transcriptase quantitative polymerase chain reaction), membrane disrupting effect (by LIVE/DEAD BacLight™ Kit), and finally antibiofilm activity (by the crystal violet assay). Both strains of S. aureus were susceptible to N. sativa EO, thymoquinone, and carvacrol. N. sativa EO and carvacrol induced the increase of EtBr accumulated by both S. aureus strains. Membrane integrity of ATCC strain was disrupted by carvacrol and p-cymene, whereas for the methicillin resistant S. aureus (MRSA) strain the membrane integrity was disrupted by each compound. N. sativa EO and its bioactive compounds such as carvacrol and p-cymene could be applied as resistance modifiers in MRSA strains.

Original languageEnglish
JournalPhytotherapy Research
DOIs
Publication statusAccepted/In press - jan. 1 2019

Fingerprint

Volatile Oils
Staphylococcus aureus
Methicillin Resistance
Ethidium
Membranes
Gentian Violet
Methicillin
Methicillin-Resistant Staphylococcus aureus
caraway oil
carvacrol
Reverse Transcriptase Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
4-cymene
Gene Expression
thymoquinone

Keywords

    ASJC Scopus subject areas

    • Pharmacology

    Cite this

    Nigella sativa essential oil and its bioactive compounds as resistance modifiers against Staphylococcus aureus. / Mouwakeh, Ahmad; Kincses, Annamária; Nové, Márta; Mosolygó, Tímea; Mohácsi-Farkas, Csilla; Kiskó, Gabriella; Spengler, G.

    In: Phytotherapy Research, 01.01.2019.

    Research output: Article

    Mouwakeh, Ahmad ; Kincses, Annamária ; Nové, Márta ; Mosolygó, Tímea ; Mohácsi-Farkas, Csilla ; Kiskó, Gabriella ; Spengler, G. / Nigella sativa essential oil and its bioactive compounds as resistance modifiers against Staphylococcus aureus. In: Phytotherapy Research. 2019.
    @article{0d8bd7dd7fa1461788e382058cc506ab,
    title = "Nigella sativa essential oil and its bioactive compounds as resistance modifiers against Staphylococcus aureus",
    abstract = "Nigella sativa essential oil (EO) and its compounds (thymoquinone, carvacrol, and p-cymene) have a broad antimicrobial spectrum. The aim of this study was to investigate the antimicrobial and resistance modifying activity of N. sativa EO, thymoquinone, carvacrol, and p-cymene against one methicillin susceptible and one methicillin resistant Staphylococcus aureus strain. N. sativa EO, thymoquinone, carvacrol, and p-cymene were assessed for antimicrobial activity and modulation of antimicrobial resistance (by broth microdilution), inhibition of antimicrobial efflux (by ethidium bromide [EtBr] accumulation assay), relative expression of mepA gene (by real-time reverse transcriptase quantitative polymerase chain reaction), membrane disrupting effect (by LIVE/DEAD BacLight™ Kit), and finally antibiofilm activity (by the crystal violet assay). Both strains of S. aureus were susceptible to N. sativa EO, thymoquinone, and carvacrol. N. sativa EO and carvacrol induced the increase of EtBr accumulated by both S. aureus strains. Membrane integrity of ATCC strain was disrupted by carvacrol and p-cymene, whereas for the methicillin resistant S. aureus (MRSA) strain the membrane integrity was disrupted by each compound. N. sativa EO and its bioactive compounds such as carvacrol and p-cymene could be applied as resistance modifiers in MRSA strains.",
    keywords = "antibacterial, biofilm, efflux pump inhibitor (EPI), Nigella sativa essential oil, Staphylococcus aureus",
    author = "Ahmad Mouwakeh and Annam{\'a}ria Kincses and M{\'a}rta Nov{\'e} and T{\'i}mea Mosolyg{\'o} and Csilla Moh{\'a}csi-Farkas and Gabriella Kisk{\'o} and G. Spengler",
    year = "2019",
    month = "1",
    day = "1",
    doi = "10.1002/ptr.6294",
    language = "English",
    journal = "Phytotherapy Research",
    issn = "0951-418X",
    publisher = "John Wiley and Sons Ltd",

    }

    TY - JOUR

    T1 - Nigella sativa essential oil and its bioactive compounds as resistance modifiers against Staphylococcus aureus

    AU - Mouwakeh, Ahmad

    AU - Kincses, Annamária

    AU - Nové, Márta

    AU - Mosolygó, Tímea

    AU - Mohácsi-Farkas, Csilla

    AU - Kiskó, Gabriella

    AU - Spengler, G.

    PY - 2019/1/1

    Y1 - 2019/1/1

    N2 - Nigella sativa essential oil (EO) and its compounds (thymoquinone, carvacrol, and p-cymene) have a broad antimicrobial spectrum. The aim of this study was to investigate the antimicrobial and resistance modifying activity of N. sativa EO, thymoquinone, carvacrol, and p-cymene against one methicillin susceptible and one methicillin resistant Staphylococcus aureus strain. N. sativa EO, thymoquinone, carvacrol, and p-cymene were assessed for antimicrobial activity and modulation of antimicrobial resistance (by broth microdilution), inhibition of antimicrobial efflux (by ethidium bromide [EtBr] accumulation assay), relative expression of mepA gene (by real-time reverse transcriptase quantitative polymerase chain reaction), membrane disrupting effect (by LIVE/DEAD BacLight™ Kit), and finally antibiofilm activity (by the crystal violet assay). Both strains of S. aureus were susceptible to N. sativa EO, thymoquinone, and carvacrol. N. sativa EO and carvacrol induced the increase of EtBr accumulated by both S. aureus strains. Membrane integrity of ATCC strain was disrupted by carvacrol and p-cymene, whereas for the methicillin resistant S. aureus (MRSA) strain the membrane integrity was disrupted by each compound. N. sativa EO and its bioactive compounds such as carvacrol and p-cymene could be applied as resistance modifiers in MRSA strains.

    AB - Nigella sativa essential oil (EO) and its compounds (thymoquinone, carvacrol, and p-cymene) have a broad antimicrobial spectrum. The aim of this study was to investigate the antimicrobial and resistance modifying activity of N. sativa EO, thymoquinone, carvacrol, and p-cymene against one methicillin susceptible and one methicillin resistant Staphylococcus aureus strain. N. sativa EO, thymoquinone, carvacrol, and p-cymene were assessed for antimicrobial activity and modulation of antimicrobial resistance (by broth microdilution), inhibition of antimicrobial efflux (by ethidium bromide [EtBr] accumulation assay), relative expression of mepA gene (by real-time reverse transcriptase quantitative polymerase chain reaction), membrane disrupting effect (by LIVE/DEAD BacLight™ Kit), and finally antibiofilm activity (by the crystal violet assay). Both strains of S. aureus were susceptible to N. sativa EO, thymoquinone, and carvacrol. N. sativa EO and carvacrol induced the increase of EtBr accumulated by both S. aureus strains. Membrane integrity of ATCC strain was disrupted by carvacrol and p-cymene, whereas for the methicillin resistant S. aureus (MRSA) strain the membrane integrity was disrupted by each compound. N. sativa EO and its bioactive compounds such as carvacrol and p-cymene could be applied as resistance modifiers in MRSA strains.

    KW - antibacterial

    KW - biofilm

    KW - efflux pump inhibitor (EPI)

    KW - Nigella sativa essential oil

    KW - Staphylococcus aureus

    UR - http://www.scopus.com/inward/record.url?scp=85060529567&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=85060529567&partnerID=8YFLogxK

    U2 - 10.1002/ptr.6294

    DO - 10.1002/ptr.6294

    M3 - Article

    JO - Phytotherapy Research

    JF - Phytotherapy Research

    SN - 0951-418X

    ER -