An HPLC method with UV and radioactivity detection is reported for analysis of the toxic metabolite of acetaminophen (paracetamol) in human liver samples. Acetaminophen and its oxidative metabolites were separated on on RP-18 column with aqueous NH4-ocetote:methanol mobile phase. The 14C-acetaminophen and its metabolites were monitored at 250 nm and by online-coupled radioactivity detection. HPLC-MS indicated the presence of the toxic metabolite - the new method proving suitable for its serial analysis.
|Publication status||Published - júl. 25 2002|
ASJC Scopus subject areas
- Analytical Chemistry
- Clinical Biochemistry
- Organic Chemistry