The preparation and optical study of 4-amino-4′-isocyanobiphenyl (ICAB) and its mono-and dialkylated derivatives (monoMICAB and diMICAB) are reported. They were found to be effective blue light emitters with a solvatochromic range of Δλmax = 104 nm, 92 nm and 90 nm, respectively. All three compounds turned out to be promising candidates in protein-labeling, yielding ∼70-190× increase in their fluorescence intensity when mixed with 10× molar excess of bovine serum albumin (BSA). The binding constants for the BSA-fluorophore complexes were found to be KICAB-BSA = 3.8 × 104 ± 5.7 × 103 M-1, KmonoMICAB-BSA = 6.0 × 104 ± 3.9 × 103 M-1 and KdiMICAB-BSA = 2.3 × 104 ± 3.2 × 103 M-1. Time resolved fluorescence measurements revealed a nearly uniform ∼2 ns lifetime for all three BSA-complexed ICAB derivatives.
|Number of pages||11|
|Journal||Journal of Photochemistry and Photobiology A: Chemistry|
|Publication status||Published - márc. 1 2016|
ASJC Scopus subject areas
- Chemical Engineering(all)
- Physics and Astronomy(all)