Nebovírus (Caliciviridae) elso hazai kimutatása hasmenéses borjú bélsármintájá ból

Pankovics Péter, Boros Ákos, Nemes Csaba, Eric Delwart, Reuter Gábor

Research output: Article

2 Citations (Scopus)

Abstract

Neboviruses are the most recent members of family Calicivirdae, in genus Nebovirus, which can be etiological agents of diarrhoea and enteric diseases of calves {Bos taurus). The genus has two prototypes: the Newbury-1 (Bo/Newbury1/1976/UK, DQ013304) and the Nebraska (BEC/NB/80/US, AY082891) viruses. Based on the viral capsid protein (VP1), neboviruses are classified into 4 phylogenetic lineages (lineage 1-4). The authors aims were the detection, genetic characterization and phylogenetic classification of neboviruses from 1-1 faecal samples collected from diarrhoeic calves living in two different cattle farms in Hungary. After the routine diagnostic bacteriological and virological investigation the detection and complete genome determination of nebovirus were done by molecular biological methods (454-pyrosequencing, RT-PCR, 573' RACE RT-PCR and direct chain terminated sequencing). Using pyrosequencing method the contigs of nebovirus sequences were aligned from one of the faecal samples and the complete viral genome was expanded with the help of additional 4 RT-PCR reactions. The genome of strain Bo/M3641/2011/HUN (JX018212) nebovirus - 5'UTR (74nt)/ ORF1 (6630nt)/ ORF2 (677nt)/3'UTR (67nt) - was 7453nt in length and it has a close to 95% nucleotide match with strain Bo/BEC/Penrith140/2000/ UK virus. Strain Bo/M3641/2011/HUN virus can phylogenetically be classified into lineage 1; Newbury-1-like phylogenetic group. In this study, the authors report the first detection, characterization and phylogenetic analysis of nebovirus in Hungary, being supposedly pathogenic agents, have already been present in Hungarian cattle herds and may contribute to calves' diarrheic diseases of unknown origin.

Original languageHungarian
Pages (from-to)12-17
Number of pages6
JournalMagyar Allatorvosok Lapja
Volume135
Issue number1
Publication statusPublished - jan. 1 2013

ASJC Scopus subject areas

  • veterinary(all)

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