Nanoparticles for intravascular applications: Pcharacterization and cytotoxicity testing

Jasmin Matuszak, Jens Baumgartner, Jan Zaloga, Maya Juenet, Acarília Eduardo Da Silva, Danielle Franke, Gunter Almer, Isabelle Texier, Damien Faivre, Josbert M. Metselaar, Fabrice P. Navarro, Cédric Chauvierre, Ruth Prassl, László Dézsi, Rudolf Urbanics, Christoph Alexiou, Harald Mangge, J. Szebeni, Didier Letourneur, Iwona Cicha

Research output: Article

29 Citations (Scopus)

Abstract

Aim: We report the physicochemical analysis of nanosystems intended for cardiovascular applications and their toxicological characterization in static and dynamic cell culture conditions. Methods: Size, polydispersity and ζ-potential were determined in 10 nanoparticle systems including liposomes, lipid nanoparticles, polymeric and iron oxide nanoparticles. Nanoparticle effects on primary human endothelial cell viability were monitored using real-time cell analysis and live-cell microscopy in static conditions, and in a flow model of arterial bifurcations. Results & conclusions: The majority of tested nanosystems were well tolerated by endothelial cells up to the concentration of 100 μg/ml in static, and up to 400 μg/ml in dynamic conditions. Pilot experiments in a pig model showed that intravenous administration of liposomal nanoparticles did not evoke the hypersensitivity reaction. These findings are of importance for future clinical use of nanosystems intended for intravascular applications.

Original languageEnglish
Pages (from-to)597-616
Number of pages20
JournalNanomedicine
Volume11
Issue number6
DOIs
Publication statusPublished - márc. 1 2016

Fingerprint

Cytotoxicity
Nanoparticles
Nanosystems
Testing
Endothelial cells
Endothelial Cells
experiment
Liposomes
Polydispersity
bifurcation
Iron oxides
Cell culture
pig
Intravenous Administration
Toxicology
iron oxide
Lipids
microscopy
Microscopy
Cell Survival

ASJC Scopus subject areas

  • Materials Science(all)
  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Development

Cite this

Matuszak, J., Baumgartner, J., Zaloga, J., Juenet, M., Da Silva, A. E., Franke, D., ... Cicha, I. (2016). Nanoparticles for intravascular applications: Pcharacterization and cytotoxicity testing. Nanomedicine, 11(6), 597-616. https://doi.org/10.2217/nnm.15.216

Nanoparticles for intravascular applications : Pcharacterization and cytotoxicity testing. / Matuszak, Jasmin; Baumgartner, Jens; Zaloga, Jan; Juenet, Maya; Da Silva, Acarília Eduardo; Franke, Danielle; Almer, Gunter; Texier, Isabelle; Faivre, Damien; Metselaar, Josbert M.; Navarro, Fabrice P.; Chauvierre, Cédric; Prassl, Ruth; Dézsi, László; Urbanics, Rudolf; Alexiou, Christoph; Mangge, Harald; Szebeni, J.; Letourneur, Didier; Cicha, Iwona.

In: Nanomedicine, Vol. 11, No. 6, 01.03.2016, p. 597-616.

Research output: Article

Matuszak, J, Baumgartner, J, Zaloga, J, Juenet, M, Da Silva, AE, Franke, D, Almer, G, Texier, I, Faivre, D, Metselaar, JM, Navarro, FP, Chauvierre, C, Prassl, R, Dézsi, L, Urbanics, R, Alexiou, C, Mangge, H, Szebeni, J, Letourneur, D & Cicha, I 2016, 'Nanoparticles for intravascular applications: Pcharacterization and cytotoxicity testing', Nanomedicine, vol. 11, no. 6, pp. 597-616. https://doi.org/10.2217/nnm.15.216
Matuszak J, Baumgartner J, Zaloga J, Juenet M, Da Silva AE, Franke D et al. Nanoparticles for intravascular applications: Pcharacterization and cytotoxicity testing. Nanomedicine. 2016 márc. 1;11(6):597-616. https://doi.org/10.2217/nnm.15.216
Matuszak, Jasmin ; Baumgartner, Jens ; Zaloga, Jan ; Juenet, Maya ; Da Silva, Acarília Eduardo ; Franke, Danielle ; Almer, Gunter ; Texier, Isabelle ; Faivre, Damien ; Metselaar, Josbert M. ; Navarro, Fabrice P. ; Chauvierre, Cédric ; Prassl, Ruth ; Dézsi, László ; Urbanics, Rudolf ; Alexiou, Christoph ; Mangge, Harald ; Szebeni, J. ; Letourneur, Didier ; Cicha, Iwona. / Nanoparticles for intravascular applications : Pcharacterization and cytotoxicity testing. In: Nanomedicine. 2016 ; Vol. 11, No. 6. pp. 597-616.
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