Myeloid but not plasmacytoid blood DCs possess Th1 polarizing and Th1/Th17 recruiting capacity in psoriasis

Ahmad Khasawneh, S. Baráth, Barbara Medgyesi, Gabriella Béke, Zsolt Dajnoki, Krisztián Gáspár, Adrienn Jenei, Lilla Pogácsás, Kitti Pázmándi, J. Gaál, Attila Bácsi, A. Szegedi, A. Kapitány

Research output: Article

2 Citations (Scopus)

Abstract

Psoriasis is a common inflammatory skin disease and dendritic cells (DCs) play crucial role in the development of skin inflammation. Although the characteristics of skin DCs in psoriasis are well defined, less is known about their peripheral blood precursors. Our aim was to characterize the phenotypic features as well as the cytokine and chemokine production of CD1c+ myeloid DCs (mDCs) and plasmacytoid DCs (pDCs) in the blood samples of psoriatic patients. Blood DCs were isolated by using a magnetic separation kit, and their intracytoplasmic cytokine production and CD83/CD86 maturation/activation marker expression were investigated by 8-colour flow cytometry. In CD1c+ mDCs the intracellular productions of Th1, Th2, Th17, Th22 and Treg polarizing cytokines were examined simultaneously, whereas in pDCs the amounts of IFNα as well as IL-12, IL-23 and IL-6 were investigated. The chemokine production of both DC populations was investigated by flow-cytometry and ELISA. According to our results psoriatic CD1c+ mDCs were in a premature state since their CD83/CD86 maturation/activation marker expression, IL-12 cytokine, CXCL9 and CCL20 chemokine production was significantly higher compared to control cells. On the other hand, blood pDCs neither produced any of the investigated cytokines and chemokines nor expressed CD83/CD86 maturation/activation markers. Our results indicate that in psoriasis not only skin but also blood mDCs perform Th1 polarizing and Th1/Th17 recruiting capacity, while pDCs function only in the skin milieu.

Original languageEnglish
Pages (from-to)109-113
Number of pages5
JournalImmunology Letters
Volume189
DOIs
Publication statusPublished - szept. 1 2017

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Psoriasis
Dendritic Cells
Blood Cells
Cytokines
Chemokines
Langerhans Cells
Interleukin-12
Skin
Chemokine CCL20
Chemokine CXCL9
Flow Cytometry
Interleukin-23
Myeloid Cells
Skin Diseases
Interleukin-6
Color
Enzyme-Linked Immunosorbent Assay
Inflammation
Population

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Myeloid but not plasmacytoid blood DCs possess Th1 polarizing and Th1/Th17 recruiting capacity in psoriasis. / Khasawneh, Ahmad; Baráth, S.; Medgyesi, Barbara; Béke, Gabriella; Dajnoki, Zsolt; Gáspár, Krisztián; Jenei, Adrienn; Pogácsás, Lilla; Pázmándi, Kitti; Gaál, J.; Bácsi, Attila; Szegedi, A.; Kapitány, A.

In: Immunology Letters, Vol. 189, 01.09.2017, p. 109-113.

Research output: Article

Khasawneh, A, Baráth, S, Medgyesi, B, Béke, G, Dajnoki, Z, Gáspár, K, Jenei, A, Pogácsás, L, Pázmándi, K, Gaál, J, Bácsi, A, Szegedi, A & Kapitány, A 2017, 'Myeloid but not plasmacytoid blood DCs possess Th1 polarizing and Th1/Th17 recruiting capacity in psoriasis', Immunology Letters, vol. 189, pp. 109-113. https://doi.org/10.1016/j.imlet.2017.04.005
Khasawneh, Ahmad ; Baráth, S. ; Medgyesi, Barbara ; Béke, Gabriella ; Dajnoki, Zsolt ; Gáspár, Krisztián ; Jenei, Adrienn ; Pogácsás, Lilla ; Pázmándi, Kitti ; Gaál, J. ; Bácsi, Attila ; Szegedi, A. ; Kapitány, A. / Myeloid but not plasmacytoid blood DCs possess Th1 polarizing and Th1/Th17 recruiting capacity in psoriasis. In: Immunology Letters. 2017 ; Vol. 189. pp. 109-113.
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abstract = "Psoriasis is a common inflammatory skin disease and dendritic cells (DCs) play crucial role in the development of skin inflammation. Although the characteristics of skin DCs in psoriasis are well defined, less is known about their peripheral blood precursors. Our aim was to characterize the phenotypic features as well as the cytokine and chemokine production of CD1c+ myeloid DCs (mDCs) and plasmacytoid DCs (pDCs) in the blood samples of psoriatic patients. Blood DCs were isolated by using a magnetic separation kit, and their intracytoplasmic cytokine production and CD83/CD86 maturation/activation marker expression were investigated by 8-colour flow cytometry. In CD1c+ mDCs the intracellular productions of Th1, Th2, Th17, Th22 and Treg polarizing cytokines were examined simultaneously, whereas in pDCs the amounts of IFNα as well as IL-12, IL-23 and IL-6 were investigated. The chemokine production of both DC populations was investigated by flow-cytometry and ELISA. According to our results psoriatic CD1c+ mDCs were in a premature state since their CD83/CD86 maturation/activation marker expression, IL-12 cytokine, CXCL9 and CCL20 chemokine production was significantly higher compared to control cells. On the other hand, blood pDCs neither produced any of the investigated cytokines and chemokines nor expressed CD83/CD86 maturation/activation markers. Our results indicate that in psoriasis not only skin but also blood mDCs perform Th1 polarizing and Th1/Th17 recruiting capacity, while pDCs function only in the skin milieu.",
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T1 - Myeloid but not plasmacytoid blood DCs possess Th1 polarizing and Th1/Th17 recruiting capacity in psoriasis

AU - Khasawneh, Ahmad

AU - Baráth, S.

AU - Medgyesi, Barbara

AU - Béke, Gabriella

AU - Dajnoki, Zsolt

AU - Gáspár, Krisztián

AU - Jenei, Adrienn

AU - Pogácsás, Lilla

AU - Pázmándi, Kitti

AU - Gaál, J.

AU - Bácsi, Attila

AU - Szegedi, A.

AU - Kapitány, A.

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Psoriasis is a common inflammatory skin disease and dendritic cells (DCs) play crucial role in the development of skin inflammation. Although the characteristics of skin DCs in psoriasis are well defined, less is known about their peripheral blood precursors. Our aim was to characterize the phenotypic features as well as the cytokine and chemokine production of CD1c+ myeloid DCs (mDCs) and plasmacytoid DCs (pDCs) in the blood samples of psoriatic patients. Blood DCs were isolated by using a magnetic separation kit, and their intracytoplasmic cytokine production and CD83/CD86 maturation/activation marker expression were investigated by 8-colour flow cytometry. In CD1c+ mDCs the intracellular productions of Th1, Th2, Th17, Th22 and Treg polarizing cytokines were examined simultaneously, whereas in pDCs the amounts of IFNα as well as IL-12, IL-23 and IL-6 were investigated. The chemokine production of both DC populations was investigated by flow-cytometry and ELISA. According to our results psoriatic CD1c+ mDCs were in a premature state since their CD83/CD86 maturation/activation marker expression, IL-12 cytokine, CXCL9 and CCL20 chemokine production was significantly higher compared to control cells. On the other hand, blood pDCs neither produced any of the investigated cytokines and chemokines nor expressed CD83/CD86 maturation/activation markers. Our results indicate that in psoriasis not only skin but also blood mDCs perform Th1 polarizing and Th1/Th17 recruiting capacity, while pDCs function only in the skin milieu.

AB - Psoriasis is a common inflammatory skin disease and dendritic cells (DCs) play crucial role in the development of skin inflammation. Although the characteristics of skin DCs in psoriasis are well defined, less is known about their peripheral blood precursors. Our aim was to characterize the phenotypic features as well as the cytokine and chemokine production of CD1c+ myeloid DCs (mDCs) and plasmacytoid DCs (pDCs) in the blood samples of psoriatic patients. Blood DCs were isolated by using a magnetic separation kit, and their intracytoplasmic cytokine production and CD83/CD86 maturation/activation marker expression were investigated by 8-colour flow cytometry. In CD1c+ mDCs the intracellular productions of Th1, Th2, Th17, Th22 and Treg polarizing cytokines were examined simultaneously, whereas in pDCs the amounts of IFNα as well as IL-12, IL-23 and IL-6 were investigated. The chemokine production of both DC populations was investigated by flow-cytometry and ELISA. According to our results psoriatic CD1c+ mDCs were in a premature state since their CD83/CD86 maturation/activation marker expression, IL-12 cytokine, CXCL9 and CCL20 chemokine production was significantly higher compared to control cells. On the other hand, blood pDCs neither produced any of the investigated cytokines and chemokines nor expressed CD83/CD86 maturation/activation markers. Our results indicate that in psoriasis not only skin but also blood mDCs perform Th1 polarizing and Th1/Th17 recruiting capacity, while pDCs function only in the skin milieu.

KW - Cell surface marker

KW - Chemokine

KW - Dendritic cell

KW - Psoriasis

KW - T cell polarizing cytokine

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