An on-line two-dimensional supercritical fluid chromatographic system (SFC/SFC) was constructed by utilizing a rotary valve Interface to provide independent flow control of the two dimensions. A cold trap was employed to refocus solutes from single or multiple fractional cuts, after being transferred to the second dimension. Improved performance, including time savings, was achieved with a packed capillary to open tubular column arrangement and two independently controlled pumps, compared to earlier reported single-pump open tubular column SFC/SFC and packed capillary column SFC/SFC systems. The packed capillary in the first dimension provided a rapid chemical class separation, while the open tubular column in the second dimension provided high resolution of closely related isomers.
ASJC Scopus subject areas
- Analytical Chemistry