mRNA population in the liver, kidney and brain of young and senescent mice: Analysis of in vitro translation products

Kaijiro Anzai, Chiyuki Imazato, Sataro Goto

Research output: Article

14 Citations (Scopus)

Abstract

Possible alterations in the population of poly(A)(+)mRNA during ageing were investigated by translation in vitro of poly(A)(+)mRNA from the liver, kidney and brain of male ddY mice of different ages. [35S]Methionine-labeled translation products were analysed by two-dimensional polyacrylamide gel electrophoresis followed by fluorography. A protein product with a molecular weight of 30 000 and isoelectric point of 6.5 was reproducibly observed only in the fluorograms of translation products of poly(a)(+)mRNA derived from the livers of senescent mice (24.5 months old). However, no age-related change was detected in the translation products of the kidney and brain. These results suggest that gene expression in liver cells changes at the level of the population of cytoplasmic poly(A)(+)mRNA during ageing.

Original languageEnglish
Pages (from-to)137-150
Number of pages14
JournalMechanisms of Ageing and Development
Volume23
Issue number2
DOIs
Publication statusPublished - okt. 1983

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ASJC Scopus subject areas

  • Ageing
  • Developmental Biology

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