Molecularly imprinted polymer-based electrochemical sensors for biopolymers

Frieder W. Scheller, Xiaorong Zhang, Aysu Yarman, Ulla Wollenberger, R. Gyurcsányi

Research output: Review article

8 Citations (Scopus)

Abstract

Electrochemical synthesis and signal generation dominate among the almost 1200 articles published annually on protein-imprinted polymers. Such polymers can be easily prepared directly on the electrode surface, and the polymer thickness can be precisely adjusted to the size of the target to enable its free exchange. In this architecture, the molecularly imprinted polymer (MIP) layer represents only one 'separation plate’; thus, the selectivity does not reach the values of ‘bulk’ measurements. The binding of target proteins can be detected straightforwardly by their modulating effect on the diffusional permeability of a redox marker through the thin MIP films. However, this generates an ‘overall apparent’ signal, which may include nonspecific interactions in the polymer layer and at the electrode surface. Certain targets, such as enzymes or redox active proteins, enables a more specific direct quantification of their binding to MIPs by in situ determination of the enzyme activity or direct electron transfer, respectively.

Original languageEnglish
Pages (from-to)53-59
Number of pages7
JournalCurrent Opinion in Electrochemistry
Volume14
DOIs
Publication statusPublished - ápr. 1 2019

Fingerprint

Electrochemical sensors
Biopolymers
Polymers
Proteins
Electrodes
Enzyme activity
Polymer films
Enzymes
Electrons

ASJC Scopus subject areas

  • Analytical Chemistry
  • Electrochemistry

Cite this

Molecularly imprinted polymer-based electrochemical sensors for biopolymers. / Scheller, Frieder W.; Zhang, Xiaorong; Yarman, Aysu; Wollenberger, Ulla; Gyurcsányi, R.

In: Current Opinion in Electrochemistry, Vol. 14, 01.04.2019, p. 53-59.

Research output: Review article

Scheller, Frieder W. ; Zhang, Xiaorong ; Yarman, Aysu ; Wollenberger, Ulla ; Gyurcsányi, R. / Molecularly imprinted polymer-based electrochemical sensors for biopolymers. In: Current Opinion in Electrochemistry. 2019 ; Vol. 14. pp. 53-59.
@article{fb08ab005b7f4ccd80e0901d155a9f8d,
title = "Molecularly imprinted polymer-based electrochemical sensors for biopolymers",
abstract = "Electrochemical synthesis and signal generation dominate among the almost 1200 articles published annually on protein-imprinted polymers. Such polymers can be easily prepared directly on the electrode surface, and the polymer thickness can be precisely adjusted to the size of the target to enable its free exchange. In this architecture, the molecularly imprinted polymer (MIP) layer represents only one 'separation plate’; thus, the selectivity does not reach the values of ‘bulk’ measurements. The binding of target proteins can be detected straightforwardly by their modulating effect on the diffusional permeability of a redox marker through the thin MIP films. However, this generates an ‘overall apparent’ signal, which may include nonspecific interactions in the polymer layer and at the electrode surface. Certain targets, such as enzymes or redox active proteins, enables a more specific direct quantification of their binding to MIPs by in situ determination of the enzyme activity or direct electron transfer, respectively.",
keywords = "Biomarker, Direct electron transfer, Electropolymerization, Epitope imprinting, Redox marker",
author = "Scheller, {Frieder W.} and Xiaorong Zhang and Aysu Yarman and Ulla Wollenberger and R. Gyurcs{\'a}nyi",
year = "2019",
month = "4",
day = "1",
doi = "10.1016/j.coelec.2018.12.005",
language = "English",
volume = "14",
pages = "53--59",
journal = "Current Opinion in Electrochemistry",
issn = "2451-9103",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Molecularly imprinted polymer-based electrochemical sensors for biopolymers

AU - Scheller, Frieder W.

AU - Zhang, Xiaorong

AU - Yarman, Aysu

AU - Wollenberger, Ulla

AU - Gyurcsányi, R.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Electrochemical synthesis and signal generation dominate among the almost 1200 articles published annually on protein-imprinted polymers. Such polymers can be easily prepared directly on the electrode surface, and the polymer thickness can be precisely adjusted to the size of the target to enable its free exchange. In this architecture, the molecularly imprinted polymer (MIP) layer represents only one 'separation plate’; thus, the selectivity does not reach the values of ‘bulk’ measurements. The binding of target proteins can be detected straightforwardly by their modulating effect on the diffusional permeability of a redox marker through the thin MIP films. However, this generates an ‘overall apparent’ signal, which may include nonspecific interactions in the polymer layer and at the electrode surface. Certain targets, such as enzymes or redox active proteins, enables a more specific direct quantification of their binding to MIPs by in situ determination of the enzyme activity or direct electron transfer, respectively.

AB - Electrochemical synthesis and signal generation dominate among the almost 1200 articles published annually on protein-imprinted polymers. Such polymers can be easily prepared directly on the electrode surface, and the polymer thickness can be precisely adjusted to the size of the target to enable its free exchange. In this architecture, the molecularly imprinted polymer (MIP) layer represents only one 'separation plate’; thus, the selectivity does not reach the values of ‘bulk’ measurements. The binding of target proteins can be detected straightforwardly by their modulating effect on the diffusional permeability of a redox marker through the thin MIP films. However, this generates an ‘overall apparent’ signal, which may include nonspecific interactions in the polymer layer and at the electrode surface. Certain targets, such as enzymes or redox active proteins, enables a more specific direct quantification of their binding to MIPs by in situ determination of the enzyme activity or direct electron transfer, respectively.

KW - Biomarker

KW - Direct electron transfer

KW - Electropolymerization

KW - Epitope imprinting

KW - Redox marker

UR - http://www.scopus.com/inward/record.url?scp=85060227956&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85060227956&partnerID=8YFLogxK

U2 - 10.1016/j.coelec.2018.12.005

DO - 10.1016/j.coelec.2018.12.005

M3 - Review article

AN - SCOPUS:85060227956

VL - 14

SP - 53

EP - 59

JO - Current Opinion in Electrochemistry

JF - Current Opinion in Electrochemistry

SN - 2451-9103

ER -