Molecular diversity of agriculturally important Aspergillus species

J. Varga, Ákos Juhász, F. Kevei, Zofia Kozakiewicz

Research output: Article

57 Citations (Scopus)

Abstract

Although Aspergillus species are not usually considered as serious plant pathogens, Aspergilli are frequently encountered in plant products. The most important consequence of their presence is mycotoxin contamination. The main mycotoxins produced by Aspergilli are the aflatoxins, ochratoxin A and patulin, which are produced by a variety of Aspergillus species in different plant commodities. Phylogenetic analysis of sequences of the ribosomal RNA gene cluster is useful for clarifying taxonomic relationships among toxigenic Aspergilli causing pre- and postharvest contamination of agricultural products. Molecular data has enabled us to clarify the taxonomy of black Aspergilli, A. flavus and its relatives, and sections Circumdati and Clavati, which include ochratoxin and patulin-producing species. Phylogenetically unrelated species were found to produce the same mycotoxins, indicating that mycotoxin-producing abilities of the isolates have been lost (or gained) several times during the evolution of the genus. The data also indicate that biosynthetic gene-based probes are necessary for molecular detection of these mycotoxin-producing organisms. The organisation of the biosynthetic genes of patulin and ochratoxins is unknown, although experiments are in progress in several laboratories to clarify the genetic background of biosynthesis of these mycotoxins. Identification of biosynthetic genes responsible for mycotoxin production is essential for clarifying the evolution of mycotoxin biosynthesis in Aspergilli, and to develop specific gene probes for the detection of mycotoxin-producing Aspergilli in agricultural products.

Original languageEnglish
Pages (from-to)627-640
Number of pages14
JournalEuropean Journal of Plant Pathology
Volume110
Issue number5-6
DOIs
Publication statusPublished - jún. 2004

Fingerprint

Aspergillus
mycotoxins
patulin
ochratoxins
agricultural products
genes
biosynthesis
plant products
ochratoxin A
multigene family
products and commodities
aflatoxins
plant pathogens
genetic background
ribosomal RNA
taxonomy
phylogeny
organisms

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science

Cite this

Molecular diversity of agriculturally important Aspergillus species. / Varga, J.; Juhász, Ákos; Kevei, F.; Kozakiewicz, Zofia.

In: European Journal of Plant Pathology, Vol. 110, No. 5-6, 06.2004, p. 627-640.

Research output: Article

Varga, J. ; Juhász, Ákos ; Kevei, F. ; Kozakiewicz, Zofia. / Molecular diversity of agriculturally important Aspergillus species. In: European Journal of Plant Pathology. 2004 ; Vol. 110, No. 5-6. pp. 627-640.
@article{f3514e350cc543f18f6756e50fa856fc,
title = "Molecular diversity of agriculturally important Aspergillus species",
abstract = "Although Aspergillus species are not usually considered as serious plant pathogens, Aspergilli are frequently encountered in plant products. The most important consequence of their presence is mycotoxin contamination. The main mycotoxins produced by Aspergilli are the aflatoxins, ochratoxin A and patulin, which are produced by a variety of Aspergillus species in different plant commodities. Phylogenetic analysis of sequences of the ribosomal RNA gene cluster is useful for clarifying taxonomic relationships among toxigenic Aspergilli causing pre- and postharvest contamination of agricultural products. Molecular data has enabled us to clarify the taxonomy of black Aspergilli, A. flavus and its relatives, and sections Circumdati and Clavati, which include ochratoxin and patulin-producing species. Phylogenetically unrelated species were found to produce the same mycotoxins, indicating that mycotoxin-producing abilities of the isolates have been lost (or gained) several times during the evolution of the genus. The data also indicate that biosynthetic gene-based probes are necessary for molecular detection of these mycotoxin-producing organisms. The organisation of the biosynthetic genes of patulin and ochratoxins is unknown, although experiments are in progress in several laboratories to clarify the genetic background of biosynthesis of these mycotoxins. Identification of biosynthetic genes responsible for mycotoxin production is essential for clarifying the evolution of mycotoxin biosynthesis in Aspergilli, and to develop specific gene probes for the detection of mycotoxin-producing Aspergilli in agricultural products.",
keywords = "Aflatoxin, Breeding, Ochratoxin, Patulin, Phylogenetics, Taxonomy",
author = "J. Varga and {\'A}kos Juh{\'a}sz and F. Kevei and Zofia Kozakiewicz",
year = "2004",
month = "6",
doi = "10.1023/B:EJPP.0000032402.36050.df",
language = "English",
volume = "110",
pages = "627--640",
journal = "European Journal of Plant Pathology",
issn = "0929-1873",
publisher = "Springer Netherlands",
number = "5-6",

}

TY - JOUR

T1 - Molecular diversity of agriculturally important Aspergillus species

AU - Varga, J.

AU - Juhász, Ákos

AU - Kevei, F.

AU - Kozakiewicz, Zofia

PY - 2004/6

Y1 - 2004/6

N2 - Although Aspergillus species are not usually considered as serious plant pathogens, Aspergilli are frequently encountered in plant products. The most important consequence of their presence is mycotoxin contamination. The main mycotoxins produced by Aspergilli are the aflatoxins, ochratoxin A and patulin, which are produced by a variety of Aspergillus species in different plant commodities. Phylogenetic analysis of sequences of the ribosomal RNA gene cluster is useful for clarifying taxonomic relationships among toxigenic Aspergilli causing pre- and postharvest contamination of agricultural products. Molecular data has enabled us to clarify the taxonomy of black Aspergilli, A. flavus and its relatives, and sections Circumdati and Clavati, which include ochratoxin and patulin-producing species. Phylogenetically unrelated species were found to produce the same mycotoxins, indicating that mycotoxin-producing abilities of the isolates have been lost (or gained) several times during the evolution of the genus. The data also indicate that biosynthetic gene-based probes are necessary for molecular detection of these mycotoxin-producing organisms. The organisation of the biosynthetic genes of patulin and ochratoxins is unknown, although experiments are in progress in several laboratories to clarify the genetic background of biosynthesis of these mycotoxins. Identification of biosynthetic genes responsible for mycotoxin production is essential for clarifying the evolution of mycotoxin biosynthesis in Aspergilli, and to develop specific gene probes for the detection of mycotoxin-producing Aspergilli in agricultural products.

AB - Although Aspergillus species are not usually considered as serious plant pathogens, Aspergilli are frequently encountered in plant products. The most important consequence of their presence is mycotoxin contamination. The main mycotoxins produced by Aspergilli are the aflatoxins, ochratoxin A and patulin, which are produced by a variety of Aspergillus species in different plant commodities. Phylogenetic analysis of sequences of the ribosomal RNA gene cluster is useful for clarifying taxonomic relationships among toxigenic Aspergilli causing pre- and postharvest contamination of agricultural products. Molecular data has enabled us to clarify the taxonomy of black Aspergilli, A. flavus and its relatives, and sections Circumdati and Clavati, which include ochratoxin and patulin-producing species. Phylogenetically unrelated species were found to produce the same mycotoxins, indicating that mycotoxin-producing abilities of the isolates have been lost (or gained) several times during the evolution of the genus. The data also indicate that biosynthetic gene-based probes are necessary for molecular detection of these mycotoxin-producing organisms. The organisation of the biosynthetic genes of patulin and ochratoxins is unknown, although experiments are in progress in several laboratories to clarify the genetic background of biosynthesis of these mycotoxins. Identification of biosynthetic genes responsible for mycotoxin production is essential for clarifying the evolution of mycotoxin biosynthesis in Aspergilli, and to develop specific gene probes for the detection of mycotoxin-producing Aspergilli in agricultural products.

KW - Aflatoxin

KW - Breeding

KW - Ochratoxin

KW - Patulin

KW - Phylogenetics

KW - Taxonomy

UR - http://www.scopus.com/inward/record.url?scp=3442887432&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3442887432&partnerID=8YFLogxK

U2 - 10.1023/B:EJPP.0000032402.36050.df

DO - 10.1023/B:EJPP.0000032402.36050.df

M3 - Article

AN - SCOPUS:3442887432

VL - 110

SP - 627

EP - 640

JO - European Journal of Plant Pathology

JF - European Journal of Plant Pathology

SN - 0929-1873

IS - 5-6

ER -