Molecular characterization of a beet ringspot nepovirus isolated from Begonia ricinifolia in Hungary

Szilvia Kis, Pál Salamon, Viktor Kis, G. Szittya

Research output: Article

3 Citations (Scopus)

Abstract

A nepovirus was isolated from Begonia ricinifolia showing chlorotic ringspot and line pattern symptoms. The purified virus had spherical particles of ca. 30 nm and contained a single coat protein subunit of ca. 56 kDa. The complete nucleotide sequence of the bipartite viral genome was determined. RNA 1 is 7394 nucleotides long, flanked by 5’ and 3’ untranslated regions (UTR), and followed by a 3’ poly-A tail. It contains a single 6810 nt long open reading frame (ORF), which is translated into a 255 kDa polyprotein composed of 2269 amino acids. The 4684 nt long RNA 2 has a 4053 nt long ORF which encodes a single polyprotein of 1350 amino acids with a molecular weight of 149 kDa. Sequence comparisons revealed that the virus isolated from B. ricinifolia has the highest sequence similarity to beet ringspot virus and should be considered as a strain of BRSV. This is the first report on the occurrence of BRSV in B. ricinifolia and the presence of this virus outside Scotland.

Original languageEnglish
Pages (from-to)3559-3562
Number of pages4
JournalArchives of Virology
Volume162
Issue number11
DOIs
Publication statusPublished - nov. 1 2017

Fingerprint

Begoniaceae
Nepovirus
Beta vulgaris
Hungary
Polyproteins
Viruses
Open Reading Frames
RNA
Cercopithecine Herpesvirus 1
Amino Acids
Viral Genome
5' Untranslated Regions
Protein Subunits
Capsid Proteins
Scotland
3' Untranslated Regions
Nucleotides
Molecular Weight
Messenger RNA

ASJC Scopus subject areas

  • Virology

Cite this

Molecular characterization of a beet ringspot nepovirus isolated from Begonia ricinifolia in Hungary. / Kis, Szilvia; Salamon, Pál; Kis, Viktor; Szittya, G.

In: Archives of Virology, Vol. 162, No. 11, 01.11.2017, p. 3559-3562.

Research output: Article

Kis, Szilvia ; Salamon, Pál ; Kis, Viktor ; Szittya, G. / Molecular characterization of a beet ringspot nepovirus isolated from Begonia ricinifolia in Hungary. In: Archives of Virology. 2017 ; Vol. 162, No. 11. pp. 3559-3562.
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