Molecular characterization and in Vitro synthesis of infectious RNA of a Turnip veinclearing virus isolated from Alliaria petiolata in Hungary

Tamás Tóth, Petér Gyula, Pál Salamon, Szilvia Kis, Anita Sós-Hegedus, György Szittya

Research output: Article

Abstract

A tobamovirus was isolated from leaves of Alliaria petiolata plants, showing vein-clearing, interveinal chlorosis, and moderate deformation. Host range experiments revealed a high similarity of isolate ApH both to ribgrass mosaic viruses and turnip vein-clearing viruses. The complete nucleotide sequence of the viral genome was determined. The genomic RNA is composed of 6312 nucleotides and contains four open reading frames (ORF). ORF1 is 3324 nt-long and encodes a polypeptide of about 125.3 kDa. The ORF1 encoded putative replication protein contains an Alphavirus-like methyltransferase domain. ORF2 is 4806 ntlong and encodes a polypeptide of about 182 kDa. The ORF2 encoded putative replication protein contains an RNA-dependent RNA polymerase, catalytic domain. ORF3 encodes the putative cell-to-cell movement protein with a molecular weight of 30.1 kDa. ORF4 overlaps with ORF3 and encodes the coat protein with a size of 17.5 kDa. Sequence comparisons revealed that the ApH isolate has the highest similarity to turnip vein-clearing viruses and should be considered an isolate of Turnip vein-clearing virus (TVCV). This is the first report on the occurrence of TVCV in Hungary. In vitro transcripts prepared from the full-length cDNA clone of TVCV-ApH were highly infectious and induced typical symptoms characteristic to the original isolate of the virus. Since infectious clones of TVCV-ApH and crTMV (another isolate of TVCV) markedly differed in respect to recovery phenotype in Arabidopsis thaliana, it is feasible to carry out gene exchange or mutational studies to determine viral factors responsible for the symptom recovery phenotype.

Original languageEnglish
Article numbere0224398
JournalPloS one
Volume14
Issue number10
DOIs
Publication statusPublished - jan. 1 2019

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Turnip vein-clearing virus
Alliaria petiolata
Brassica napus
turnips
Hungary
Viruses
Veins
RNA
viruses
synthesis
Ribgrass mosaic virus
signs and symptoms (plants)
polypeptides
RNA-directed RNA polymerase
Tobamovirus
clones
Clone Cells
Alphavirus
phenotype
Nucleotides

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Molecular characterization and in Vitro synthesis of infectious RNA of a Turnip veinclearing virus isolated from Alliaria petiolata in Hungary. / Tóth, Tamás; Gyula, Petér; Salamon, Pál; Kis, Szilvia; Sós-Hegedus, Anita; Szittya, György.

In: PloS one, Vol. 14, No. 10, e0224398, 01.01.2019.

Research output: Article

Tóth, Tamás ; Gyula, Petér ; Salamon, Pál ; Kis, Szilvia ; Sós-Hegedus, Anita ; Szittya, György. / Molecular characterization and in Vitro synthesis of infectious RNA of a Turnip veinclearing virus isolated from Alliaria petiolata in Hungary. In: PloS one. 2019 ; Vol. 14, No. 10.
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