Molecular analysis of S-haplotypes in peach, a self-compatible Prunus species

Attila Hegedüs, Zoltán Szabó, József Nyéki, Júlia Halász, Andrzej Pedryc

Research output: Article

9 Citations (Scopus)

Abstract

The most commercially grown peach [Prunus persica (L.) Batsch.] cultivars do not require cross-pollination for reasonable fruit set; however, self-incompatibility is a well-known feature within the Prunoideae subfamily. Isoelectric focusing and native polyacrylamide gel electrophoresis of S-ribonucleases; PCR analyses of S-RNase and S-haplotype-specific F-box genes as well as DNA sequencing were carried out to survey the self-(in)compatibility allele pool and to uncover the nature of self-compatibility in peach. From 25 cultivars and hybrids with considerable diversity in phenotype and origin, only two S-haplotypes were detected. Allele identity could be checked by exact length determination of the PCR-amplified fragments and/or partial sequencing of the peach S1-, S2-, and Prunus davidiana (Carr.) Franch. S1-RNases. S-RNases of peach were detected to possess ribonuclease activity, and a single nucleotide polymorphism in the S1-RNase was shown, which represents a synonymous substitution and does not change the amino acid present at the position in the protein. A 700-bp fragment of the peach SFB gene was PCR-amplified, which is similar to the fragment size of functional Prunus L. SFBs. All data obtained in this study may support the contribution of genes outside the S-locus to the self-compatible phenotype of peaches.

Original languageEnglish
Pages (from-to)738-743
Number of pages6
JournalJournal of the American Society for Horticultural Science
Volume131
Issue number6
Publication statusPublished - nov. 1 2006

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ASJC Scopus subject areas

  • Genetics
  • Horticulture

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