Moesin becomes linked to the plasma membrane in attached neutrophil granulocytes

Margit Keresztes, Zsuzsa Lajtos, János Fischer, László Dux

Research output: Article

9 Citations (Scopus)

Abstract

Following 35 min of adhesion to a plastic surface, an 80-kDa F-actin-binding protein was shown to be enriched in the plasma membrane fractions of porcine neutrophils by protein blotting with labeled F-actin. This protein was almost undetectable in membrane fractions of free floating neutrophils, while it was present in total cell samples. The 80-kDa protein appeared to be a major high molecular mass component of the isolated actin-cytoskeleton of both control and attached cells. The studied F-actin-binding protein was recognized by anti-moesin antibodies. Our results suggest that moesin is translocated to the plasma membrane upon adhesion of neutrophils to the extracellular surface.

Original languageEnglish
Pages (from-to)723-727
Number of pages5
JournalBiochemical and biophysical research communications
Volume252
Issue number3
DOIs
Publication statusPublished - nov. 27 1998

    Fingerprint

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this