Abstract
The particle-free crude extract of differentiated granulocytes (GCE) and the GI-1, GI-2, GI-3 proliferation inhibitory fractions (M.W. ≥ 70,000 ∼ 11,500 and ≤ 4000) were studied for the effects they exert on cultured cells. As shown by the curve, in bone marrow suspension cultures, in the dose range of 0.01-10.0 μg/ml, GCE maximally inhibits 3H-TdR incorporation into the DNA for 5.5 hours. In agar-gel cultures, 1.0-5.0 μg/ml of GCE reduces both the number (by 50.6-54.8%) and the size of the colonies formed. The manifestation of the inhibitory effect of GI-1, GI-2 and GI-3 fractions requires 2.5-3.5 hours. The inhibitors designated GI-2 and GI-3 are target-cell specific but they do not reduce 3H-TdR incorporation either in thymocyte suspension or HeLa monolayer cultures. On the other hand GI-1 inhibits the proliferation in HeLa cultures, as well. These endogenous inhibitors exert their optimum effect, even in a partially purified state at 2-4 orders of magnitude lower concentrations than does 1, 2 5,6-dianhydrogalactitol (DAD), a cell-aspecific inhibitor used for comparison in the same system.
| Original language | English |
|---|---|
| Pages (from-to) | 207-218 |
| Number of pages | 12 |
| Journal | Mechanisms of Ageing and Development |
| Volume | 6 |
| Issue number | C |
| DOIs | |
| Publication status | Published - 1977 |
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ASJC Scopus subject areas
- Ageing
- Biochemistry
- Developmental Biology
- Developmental Neuroscience
Cite this
Modulative effect of endogenous granuloid inhibitors on cell proliferation. / Balázs, A.; Klupp, T.; Zsila, G.; Blazsek, I.; Holczinger, L.; Gaál, D.
In: Mechanisms of Ageing and Development, Vol. 6, No. C, 1977, p. 207-218.Research output: Article
}
TY - JOUR
T1 - Modulative effect of endogenous granuloid inhibitors on cell proliferation
AU - Balázs, A.
AU - Klupp, T.
AU - Zsila, G.
AU - Blazsek, I.
AU - Holczinger, L.
AU - Gaál, D.
PY - 1977
Y1 - 1977
N2 - The particle-free crude extract of differentiated granulocytes (GCE) and the GI-1, GI-2, GI-3 proliferation inhibitory fractions (M.W. ≥ 70,000 ∼ 11,500 and ≤ 4000) were studied for the effects they exert on cultured cells. As shown by the curve, in bone marrow suspension cultures, in the dose range of 0.01-10.0 μg/ml, GCE maximally inhibits 3H-TdR incorporation into the DNA for 5.5 hours. In agar-gel cultures, 1.0-5.0 μg/ml of GCE reduces both the number (by 50.6-54.8%) and the size of the colonies formed. The manifestation of the inhibitory effect of GI-1, GI-2 and GI-3 fractions requires 2.5-3.5 hours. The inhibitors designated GI-2 and GI-3 are target-cell specific but they do not reduce 3H-TdR incorporation either in thymocyte suspension or HeLa monolayer cultures. On the other hand GI-1 inhibits the proliferation in HeLa cultures, as well. These endogenous inhibitors exert their optimum effect, even in a partially purified state at 2-4 orders of magnitude lower concentrations than does 1, 2 5,6-dianhydrogalactitol (DAD), a cell-aspecific inhibitor used for comparison in the same system.
AB - The particle-free crude extract of differentiated granulocytes (GCE) and the GI-1, GI-2, GI-3 proliferation inhibitory fractions (M.W. ≥ 70,000 ∼ 11,500 and ≤ 4000) were studied for the effects they exert on cultured cells. As shown by the curve, in bone marrow suspension cultures, in the dose range of 0.01-10.0 μg/ml, GCE maximally inhibits 3H-TdR incorporation into the DNA for 5.5 hours. In agar-gel cultures, 1.0-5.0 μg/ml of GCE reduces both the number (by 50.6-54.8%) and the size of the colonies formed. The manifestation of the inhibitory effect of GI-1, GI-2 and GI-3 fractions requires 2.5-3.5 hours. The inhibitors designated GI-2 and GI-3 are target-cell specific but they do not reduce 3H-TdR incorporation either in thymocyte suspension or HeLa monolayer cultures. On the other hand GI-1 inhibits the proliferation in HeLa cultures, as well. These endogenous inhibitors exert their optimum effect, even in a partially purified state at 2-4 orders of magnitude lower concentrations than does 1, 2 5,6-dianhydrogalactitol (DAD), a cell-aspecific inhibitor used for comparison in the same system.
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UR - http://www.scopus.com/inward/citedby.url?scp=0017364639&partnerID=8YFLogxK
U2 - 10.1016/0047-6374(77)90022-7
DO - 10.1016/0047-6374(77)90022-7
M3 - Article
C2 - 559224
AN - SCOPUS:0017364639
VL - 6
SP - 207
EP - 218
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
SN - 0047-6374
IS - C
ER -