MIPs and aptamers for recognition of proteins in biomimetic sensing

Marcus Menger, Aysu Yarman, Júlia Erdossy, Huseyin Bekir Yildiz, R. Gyurcsányi, Frieder W. Scheller

Research output: Review article

20 Citations (Scopus)

Abstract

Biomimetic binders and catalysts have been generated in order to substitute the biological pendants in separation techniques and bioanalysis. The two major approaches use either "evolution in the test tube" of nucleotides for the preparation of aptamers or total chemical synthesis for molecularly imprinted polymers (MIPs). The reproducible production of aptamers is a clear advantage, whilst the preparation of MIPs typically leads to a population of polymers with different binding sites. The realization of binding sites in the total bulk of the MIPs results in a higher binding capacity, however, on the expense of the accessibility and exchange rate. Furthermore, the readout of the bound analyte is easier for aptamers since the integration of signal generating labels is well established. On the other hand, the overall negative charge of the nucleotides makes aptamers prone to non-specific adsorption of positively charged constituents of the sample and the "biological" degradation of non-modified aptamers and ionic strength-dependent changes of conformation may be challenging in some application.

Original languageEnglish
Article number35
JournalBiosensors
Volume6
Issue number3
DOIs
Publication statusPublished - 2016

Fingerprint

Biomimetics
Polymers
Nucleotide Aptamers
Proteins
Binding Sites
Ionic strength
Osmolar Concentration
Adsorption
Binders
Conformations
Labels
Nucleotides
Degradation
Catalysts
Population

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Menger, M., Yarman, A., Erdossy, J., Yildiz, H. B., Gyurcsányi, R., & Scheller, F. W. (2016). MIPs and aptamers for recognition of proteins in biomimetic sensing. Biosensors, 6(3), [35]. https://doi.org/10.3390/bios6030035

MIPs and aptamers for recognition of proteins in biomimetic sensing. / Menger, Marcus; Yarman, Aysu; Erdossy, Júlia; Yildiz, Huseyin Bekir; Gyurcsányi, R.; Scheller, Frieder W.

In: Biosensors, Vol. 6, No. 3, 35, 2016.

Research output: Review article

Menger, M, Yarman, A, Erdossy, J, Yildiz, HB, Gyurcsányi, R & Scheller, FW 2016, 'MIPs and aptamers for recognition of proteins in biomimetic sensing', Biosensors, vol. 6, no. 3, 35. https://doi.org/10.3390/bios6030035
Menger, Marcus ; Yarman, Aysu ; Erdossy, Júlia ; Yildiz, Huseyin Bekir ; Gyurcsányi, R. ; Scheller, Frieder W. / MIPs and aptamers for recognition of proteins in biomimetic sensing. In: Biosensors. 2016 ; Vol. 6, No. 3.
@article{4a96ca753cad4d2495b7b2e0af420286,
title = "MIPs and aptamers for recognition of proteins in biomimetic sensing",
abstract = "Biomimetic binders and catalysts have been generated in order to substitute the biological pendants in separation techniques and bioanalysis. The two major approaches use either {"}evolution in the test tube{"} of nucleotides for the preparation of aptamers or total chemical synthesis for molecularly imprinted polymers (MIPs). The reproducible production of aptamers is a clear advantage, whilst the preparation of MIPs typically leads to a population of polymers with different binding sites. The realization of binding sites in the total bulk of the MIPs results in a higher binding capacity, however, on the expense of the accessibility and exchange rate. Furthermore, the readout of the bound analyte is easier for aptamers since the integration of signal generating labels is well established. On the other hand, the overall negative charge of the nucleotides makes aptamers prone to non-specific adsorption of positively charged constituents of the sample and the {"}biological{"} degradation of non-modified aptamers and ionic strength-dependent changes of conformation may be challenging in some application.",
keywords = "Aptamers, Aptasensors, Biomimetic recognition elements, Chemical sensors, In vitro selection, Molecularly imprinted polymers, SELEX",
author = "Marcus Menger and Aysu Yarman and J{\'u}lia Erdossy and Yildiz, {Huseyin Bekir} and R. Gyurcs{\'a}nyi and Scheller, {Frieder W.}",
year = "2016",
doi = "10.3390/bios6030035",
language = "English",
volume = "6",
journal = "Biosensors",
issn = "0956-5663",
publisher = "Multidisciplinary Digital Publishing Institute",
number = "3",

}

TY - JOUR

T1 - MIPs and aptamers for recognition of proteins in biomimetic sensing

AU - Menger, Marcus

AU - Yarman, Aysu

AU - Erdossy, Júlia

AU - Yildiz, Huseyin Bekir

AU - Gyurcsányi, R.

AU - Scheller, Frieder W.

PY - 2016

Y1 - 2016

N2 - Biomimetic binders and catalysts have been generated in order to substitute the biological pendants in separation techniques and bioanalysis. The two major approaches use either "evolution in the test tube" of nucleotides for the preparation of aptamers or total chemical synthesis for molecularly imprinted polymers (MIPs). The reproducible production of aptamers is a clear advantage, whilst the preparation of MIPs typically leads to a population of polymers with different binding sites. The realization of binding sites in the total bulk of the MIPs results in a higher binding capacity, however, on the expense of the accessibility and exchange rate. Furthermore, the readout of the bound analyte is easier for aptamers since the integration of signal generating labels is well established. On the other hand, the overall negative charge of the nucleotides makes aptamers prone to non-specific adsorption of positively charged constituents of the sample and the "biological" degradation of non-modified aptamers and ionic strength-dependent changes of conformation may be challenging in some application.

AB - Biomimetic binders and catalysts have been generated in order to substitute the biological pendants in separation techniques and bioanalysis. The two major approaches use either "evolution in the test tube" of nucleotides for the preparation of aptamers or total chemical synthesis for molecularly imprinted polymers (MIPs). The reproducible production of aptamers is a clear advantage, whilst the preparation of MIPs typically leads to a population of polymers with different binding sites. The realization of binding sites in the total bulk of the MIPs results in a higher binding capacity, however, on the expense of the accessibility and exchange rate. Furthermore, the readout of the bound analyte is easier for aptamers since the integration of signal generating labels is well established. On the other hand, the overall negative charge of the nucleotides makes aptamers prone to non-specific adsorption of positively charged constituents of the sample and the "biological" degradation of non-modified aptamers and ionic strength-dependent changes of conformation may be challenging in some application.

KW - Aptamers

KW - Aptasensors

KW - Biomimetic recognition elements

KW - Chemical sensors

KW - In vitro selection

KW - Molecularly imprinted polymers

KW - SELEX

UR - http://www.scopus.com/inward/record.url?scp=84986183423&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84986183423&partnerID=8YFLogxK

U2 - 10.3390/bios6030035

DO - 10.3390/bios6030035

M3 - Review article

C2 - 27438862

AN - SCOPUS:84986183423

VL - 6

JO - Biosensors

JF - Biosensors

SN - 0956-5663

IS - 3

M1 - 35

ER -