Minibeet initiation from derooted sugarbeet (Beta vulgaris L.) seedlings in vitro

O. Toldi, Gábor Gyulai, Éva Preininger, E. Várallyay, Miklós Fári, E. Balázs

Research output: Article

8 Citations (Scopus)

Abstract

An in vitro method for the initiation of minibeet development from derooted sugarbeet seedlings was developed. The surface sterilized seeds were germinated for 25 days on sugar-free Murashige and Skoog medium containing half-strength nutritive elements, 10 mg l-1 thiamine, 0.1 mg l-1 gibberellic acid and 7.0 g l-1 agar. After germination, seedlings of six different genotypes were derooted at the root neck and incubated for 90-120 days on minibeet initiation medium containing double-strength Murashige and Skoog elements, B5 vitamins, 100 mg l-1 activated charcoal, 30 g l-1 d-glucose, 6-benzylaminopurine (0.2 mg l-1) and abscisic acid (0.2 mg l-1). Without subculturing, minibeets developed on 20-40% of five different genotypes of seedlings. The developed minibeets were characterized structurally and functionally by means of a histological study and relative sucrose accumulation. Minibeets were compared to tuber-like formations which showed neither polycambial structure nor relative sucrose accumulation. Minibeets gave rise to plants after potting in the greenhouse.

Original languageEnglish
Pages (from-to)217-224
Number of pages8
JournalPlant Science
Volume97
Issue number2
DOIs
Publication statusPublished - 1994

Fingerprint

N-benzyladenine
Beta vulgaris
Seedlings
sugar beet
Sucrose
Pantothenic Acid
Abscisic Acid
seedlings
Charcoal
Thiamine
Greenhouses
Genotype
Sugars
sucrose
Agar
Seed
pantothenic acid
root crown
genotype
activated carbon

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

Minibeet initiation from derooted sugarbeet (Beta vulgaris L.) seedlings in vitro. / Toldi, O.; Gyulai, Gábor; Preininger, Éva; Várallyay, E.; Fári, Miklós; Balázs, E.

In: Plant Science, Vol. 97, No. 2, 1994, p. 217-224.

Research output: Article

Toldi, O. ; Gyulai, Gábor ; Preininger, Éva ; Várallyay, E. ; Fári, Miklós ; Balázs, E. / Minibeet initiation from derooted sugarbeet (Beta vulgaris L.) seedlings in vitro. In: Plant Science. 1994 ; Vol. 97, No. 2. pp. 217-224.
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AU - Balázs, E.

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