Microcapillary specifically designed for pressure microinjections of very low volumes

Research output: Article

3 Citations (Scopus)

Abstract

Tracer micronjections are very widely used in brain mapping research. While administration of small quantities/volumes of tracers can easily be achieved through iontophoresis, in the case of pressure injections the volume of the substances injected are much more difficult to control. Instead of using different variables like hydrodynamic conductance, pressure pulses, pressure-temperature-dependent protocols to quantify very small volumes in the nanoliter range out of a higher volume, within the confines of the present study a novel microcapillary design is presented. This microcapillary contains exactly the volume of tracer one intends to inject, therefore the danger of flooding large brain areas or the risk of tracer leakage to neighbouring nuclei are completely eliminated, and in the same time this design assures that very small and circumscribed areas can be labeled and their connections mapped, thus making the experiments more specific. In combination with high precision stereotaxic measurements these small volumes of tracers can yield well targeted and very discrete injection sites that make possible the mapping of individual nuclear subdivisions or delicate nuclei in the brain.

Original languageEnglish
Pages (from-to)229-234
Number of pages6
JournalJournal of Neuroscience Methods
Volume190
Issue number2
DOIs
Publication statusPublished - júl. 2010

Fingerprint

Microinjections
Brain Mapping
Pressure
Iontophoresis
Injections
Brain
Hydrodynamics
Blood Pressure
Temperature
Research

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

@article{38c410bac1124b90b513e2aea6b410f2,
title = "Microcapillary specifically designed for pressure microinjections of very low volumes",
abstract = "Tracer micronjections are very widely used in brain mapping research. While administration of small quantities/volumes of tracers can easily be achieved through iontophoresis, in the case of pressure injections the volume of the substances injected are much more difficult to control. Instead of using different variables like hydrodynamic conductance, pressure pulses, pressure-temperature-dependent protocols to quantify very small volumes in the nanoliter range out of a higher volume, within the confines of the present study a novel microcapillary design is presented. This microcapillary contains exactly the volume of tracer one intends to inject, therefore the danger of flooding large brain areas or the risk of tracer leakage to neighbouring nuclei are completely eliminated, and in the same time this design assures that very small and circumscribed areas can be labeled and their connections mapped, thus making the experiments more specific. In combination with high precision stereotaxic measurements these small volumes of tracers can yield well targeted and very discrete injection sites that make possible the mapping of individual nuclear subdivisions or delicate nuclei in the brain.",
keywords = "Anterograde tracer, Brain mapping, Microcapillary, Nanoliter, Pressure injection, Retrograde tracer, Tract tracing, Virus microinjections",
author = "Z. Kov{\'a}cs and M. Palk{\'o}vits",
year = "2010",
month = "7",
doi = "10.1016/j.jneumeth.2010.05.016",
language = "English",
volume = "190",
pages = "229--234",
journal = "Journal of Neuroscience Methods",
issn = "0165-0270",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Microcapillary specifically designed for pressure microinjections of very low volumes

AU - Kovács, Z.

AU - Palkóvits, M.

PY - 2010/7

Y1 - 2010/7

N2 - Tracer micronjections are very widely used in brain mapping research. While administration of small quantities/volumes of tracers can easily be achieved through iontophoresis, in the case of pressure injections the volume of the substances injected are much more difficult to control. Instead of using different variables like hydrodynamic conductance, pressure pulses, pressure-temperature-dependent protocols to quantify very small volumes in the nanoliter range out of a higher volume, within the confines of the present study a novel microcapillary design is presented. This microcapillary contains exactly the volume of tracer one intends to inject, therefore the danger of flooding large brain areas or the risk of tracer leakage to neighbouring nuclei are completely eliminated, and in the same time this design assures that very small and circumscribed areas can be labeled and their connections mapped, thus making the experiments more specific. In combination with high precision stereotaxic measurements these small volumes of tracers can yield well targeted and very discrete injection sites that make possible the mapping of individual nuclear subdivisions or delicate nuclei in the brain.

AB - Tracer micronjections are very widely used in brain mapping research. While administration of small quantities/volumes of tracers can easily be achieved through iontophoresis, in the case of pressure injections the volume of the substances injected are much more difficult to control. Instead of using different variables like hydrodynamic conductance, pressure pulses, pressure-temperature-dependent protocols to quantify very small volumes in the nanoliter range out of a higher volume, within the confines of the present study a novel microcapillary design is presented. This microcapillary contains exactly the volume of tracer one intends to inject, therefore the danger of flooding large brain areas or the risk of tracer leakage to neighbouring nuclei are completely eliminated, and in the same time this design assures that very small and circumscribed areas can be labeled and their connections mapped, thus making the experiments more specific. In combination with high precision stereotaxic measurements these small volumes of tracers can yield well targeted and very discrete injection sites that make possible the mapping of individual nuclear subdivisions or delicate nuclei in the brain.

KW - Anterograde tracer

KW - Brain mapping

KW - Microcapillary

KW - Nanoliter

KW - Pressure injection

KW - Retrograde tracer

KW - Tract tracing

KW - Virus microinjections

UR - http://www.scopus.com/inward/record.url?scp=77954219741&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954219741&partnerID=8YFLogxK

U2 - 10.1016/j.jneumeth.2010.05.016

DO - 10.1016/j.jneumeth.2010.05.016

M3 - Article

C2 - 20576508

AN - SCOPUS:77954219741

VL - 190

SP - 229

EP - 234

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 0165-0270

IS - 2

ER -