Methodological guidelines to study extracellular vesicles

Frank A.W. Coumans, Alain R. Brisson, E. Búzás, Françoise Dignat-George, Esther E.E. Drees, Samir El-Andaloussi, Costanza Emanueli, Aleksandra Gasecka, An Hendrix, Andrew F. Hill, Romaric Lacroix, Yi Lee, Ton G. Van Leeuwen, Nigel Mackman, Imre Mäger, John P. Nolan, Edwin Van Der Pol, D. Michiel Pegtel, Susmita Sahoo, Pia R.M. SiljanderGuus Sturk, Olivier De Wever, Rienk Nieuwland

Research output: Review article

170 Citations (Scopus)

Abstract

Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.

Original languageEnglish
Pages (from-to)1632-1648
Number of pages17
JournalCirculation Research
Volume120
Issue number10
DOIs
Publication statusPublished - máj. 12 2017

Fingerprint

Guidelines
Body Fluids
Extracellular Vesicles
Research

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Coumans, F. A. W., Brisson, A. R., Búzás, E., Dignat-George, F., Drees, E. E. E., El-Andaloussi, S., ... Nieuwland, R. (2017). Methodological guidelines to study extracellular vesicles. Circulation Research, 120(10), 1632-1648. https://doi.org/10.1161/CIRCRESAHA.117.309417

Methodological guidelines to study extracellular vesicles. / Coumans, Frank A.W.; Brisson, Alain R.; Búzás, E.; Dignat-George, Françoise; Drees, Esther E.E.; El-Andaloussi, Samir; Emanueli, Costanza; Gasecka, Aleksandra; Hendrix, An; Hill, Andrew F.; Lacroix, Romaric; Lee, Yi; Van Leeuwen, Ton G.; Mackman, Nigel; Mäger, Imre; Nolan, John P.; Van Der Pol, Edwin; Pegtel, D. Michiel; Sahoo, Susmita; Siljander, Pia R.M.; Sturk, Guus; De Wever, Olivier; Nieuwland, Rienk.

In: Circulation Research, Vol. 120, No. 10, 12.05.2017, p. 1632-1648.

Research output: Review article

Coumans, FAW, Brisson, AR, Búzás, E, Dignat-George, F, Drees, EEE, El-Andaloussi, S, Emanueli, C, Gasecka, A, Hendrix, A, Hill, AF, Lacroix, R, Lee, Y, Van Leeuwen, TG, Mackman, N, Mäger, I, Nolan, JP, Van Der Pol, E, Pegtel, DM, Sahoo, S, Siljander, PRM, Sturk, G, De Wever, O & Nieuwland, R 2017, 'Methodological guidelines to study extracellular vesicles', Circulation Research, vol. 120, no. 10, pp. 1632-1648. https://doi.org/10.1161/CIRCRESAHA.117.309417
Coumans FAW, Brisson AR, Búzás E, Dignat-George F, Drees EEE, El-Andaloussi S et al. Methodological guidelines to study extracellular vesicles. Circulation Research. 2017 máj. 12;120(10):1632-1648. https://doi.org/10.1161/CIRCRESAHA.117.309417
Coumans, Frank A.W. ; Brisson, Alain R. ; Búzás, E. ; Dignat-George, Françoise ; Drees, Esther E.E. ; El-Andaloussi, Samir ; Emanueli, Costanza ; Gasecka, Aleksandra ; Hendrix, An ; Hill, Andrew F. ; Lacroix, Romaric ; Lee, Yi ; Van Leeuwen, Ton G. ; Mackman, Nigel ; Mäger, Imre ; Nolan, John P. ; Van Der Pol, Edwin ; Pegtel, D. Michiel ; Sahoo, Susmita ; Siljander, Pia R.M. ; Sturk, Guus ; De Wever, Olivier ; Nieuwland, Rienk. / Methodological guidelines to study extracellular vesicles. In: Circulation Research. 2017 ; Vol. 120, No. 10. pp. 1632-1648.
@article{9661faaa142140d180ca15162cdd0178,
title = "Methodological guidelines to study extracellular vesicles",
abstract = "Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.",
keywords = "cardiovascular diseases, exosomes, extracellular vesicles, methods, reference standards",
author = "Coumans, {Frank A.W.} and Brisson, {Alain R.} and E. B{\'u}z{\'a}s and Fran{\cc}oise Dignat-George and Drees, {Esther E.E.} and Samir El-Andaloussi and Costanza Emanueli and Aleksandra Gasecka and An Hendrix and Hill, {Andrew F.} and Romaric Lacroix and Yi Lee and {Van Leeuwen}, {Ton G.} and Nigel Mackman and Imre M{\"a}ger and Nolan, {John P.} and {Van Der Pol}, Edwin and Pegtel, {D. Michiel} and Susmita Sahoo and Siljander, {Pia R.M.} and Guus Sturk and {De Wever}, Olivier and Rienk Nieuwland",
year = "2017",
month = "5",
day = "12",
doi = "10.1161/CIRCRESAHA.117.309417",
language = "English",
volume = "120",
pages = "1632--1648",
journal = "Circulation Research",
issn = "0009-7330",
publisher = "Lippincott Williams and Wilkins",
number = "10",

}

TY - JOUR

T1 - Methodological guidelines to study extracellular vesicles

AU - Coumans, Frank A.W.

AU - Brisson, Alain R.

AU - Búzás, E.

AU - Dignat-George, Françoise

AU - Drees, Esther E.E.

AU - El-Andaloussi, Samir

AU - Emanueli, Costanza

AU - Gasecka, Aleksandra

AU - Hendrix, An

AU - Hill, Andrew F.

AU - Lacroix, Romaric

AU - Lee, Yi

AU - Van Leeuwen, Ton G.

AU - Mackman, Nigel

AU - Mäger, Imre

AU - Nolan, John P.

AU - Van Der Pol, Edwin

AU - Pegtel, D. Michiel

AU - Sahoo, Susmita

AU - Siljander, Pia R.M.

AU - Sturk, Guus

AU - De Wever, Olivier

AU - Nieuwland, Rienk

PY - 2017/5/12

Y1 - 2017/5/12

N2 - Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.

AB - Owing to the relationship between extracellular vesicles (EVs) and physiological and pathological conditions, the interest in EVs is exponentially growing. EVs hold high hopes for novel diagnostic and translational discoveries. This review provides an expert-based update of recent advances in the methods to study EVs and summarizes currently accepted considerations and recommendations from sample collection to isolation, detection, and characterization of EVs. Common misconceptions and methodological pitfalls are highlighted. Although EVs are found in all body fluids, in this review, we will focus on EVs from human blood, not only our most complex but also the most interesting body fluid for cardiovascular research.

KW - cardiovascular diseases

KW - exosomes

KW - extracellular vesicles

KW - methods

KW - reference standards

UR - http://www.scopus.com/inward/record.url?scp=85019953265&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85019953265&partnerID=8YFLogxK

U2 - 10.1161/CIRCRESAHA.117.309417

DO - 10.1161/CIRCRESAHA.117.309417

M3 - Review article

C2 - 28495994

AN - SCOPUS:85019953265

VL - 120

SP - 1632

EP - 1648

JO - Circulation Research

JF - Circulation Research

SN - 0009-7330

IS - 10

ER -