Metal ion-induced conformational changes of phosphorylated fragments of human neurofilament (NF-M) protein

M. Hollósi, L. Ürge, A. Perczel, J. Kajtár, I. Teplán, L. Ötvös, G. D. Fasman

Research output: Article

62 Citations (Scopus)

Abstract

The NF-M subunit of human neurofilaments has a C-terminal repeating 13-mer sequence. The 13-mer (Lys-Ser-Pro-Val-Pro-Lys-Ser-Pro-Val-Glu-Glu-Lys-Gly) (NF-M13) and 17-mer (Glu-Glu-Lys-Gly)-(NF-M13) sequences were synthesized, as were both the mono- and diphosphorylated Ser species. Circular dichroism (c.d.) studies and c.d. titrations with Al3+ and Ca2+ were performed. The conformation of the phosphorylated and unphosphorylated material was random in water. Deconvolution of the c.d. spectra, in trifluoroethanol, of the untitrated samples yielded a high content of unordered structure, similar to the poly-l-proline II structure. Titration of the phosphorylated species with Al3+ or Ca2+ caused a surprising conformational change to occur, yielding a high content of β-pleated sheet structure. A mechanism of metal binding to the phosphofragments is proposed which may be relevant to the formation of neurofibrillary tangles in Alzheimer's disease.

Original languageEnglish
Pages (from-to)673-682
Number of pages10
JournalJournal of Molecular Biology
Volume223
Issue number3
DOIs
Publication statusPublished - febr. 5 1992

Fingerprint

Neurofilament Proteins
Circular Dichroism
Intermediate Filaments
Metals
Ions
Trifluoroethanol
Neurofibrillary Tangles
Proline
Alzheimer Disease
Water

ASJC Scopus subject areas

  • Virology

Cite this

Metal ion-induced conformational changes of phosphorylated fragments of human neurofilament (NF-M) protein. / Hollósi, M.; Ürge, L.; Perczel, A.; Kajtár, J.; Teplán, I.; Ötvös, L.; Fasman, G. D.

In: Journal of Molecular Biology, Vol. 223, No. 3, 05.02.1992, p. 673-682.

Research output: Article

Hollósi, M. ; Ürge, L. ; Perczel, A. ; Kajtár, J. ; Teplán, I. ; Ötvös, L. ; Fasman, G. D. / Metal ion-induced conformational changes of phosphorylated fragments of human neurofilament (NF-M) protein. In: Journal of Molecular Biology. 1992 ; Vol. 223, No. 3. pp. 673-682.
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AU - Teplán, I.

AU - Ötvös, L.

AU - Fasman, G. D.

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