Metabolite analysis, isolation and purity assessment using various liquid chromatographic techniques combined with radioactivity detection

Gy Morovján, B. Dalmadi-Kiss, I. Klebovich, E. Mincsovics

Research output: Article

20 Citations (Scopus)

Abstract

During the discovery of metabolic routes of a drug candidate, radioactively labeled substances are administered. This study reports the multidimensional application of overpressured layer chromatography (OPLC) and high-performance liquid chromatography (HPLC) coupled with online or off-line nondestructive radioactivity detection methods in metabolism studies. Among these methods, digital autoradiography and flow-cell radioactivity detectors (RD) using solid scintillators are used. In this study, the hyphenation of OPLC with RD is reported. The application of the OPLC-RD technique is demonstrated on a metabolism study as well as the multidimensional chromatographic selectivity using normal-phase OPLC for the separation in the first dimension, followed by reversed-phase HPLC-RD, which provides additional selectivity to the separation. Information regarding the identity of radiolabeled metabolites and data obtained from spectroscopic methods could be advantageously used during structure elucidation.

Original languageEnglish
Pages (from-to)603-608
Number of pages6
JournalJournal of chromatographic science
Volume40
Issue number10
DOIs
Publication statusPublished - jan. 1 2002

ASJC Scopus subject areas

  • Analytical Chemistry

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