Measurement of the modification and interference rate of urinary albumin detected by size-exclusion HPLC

Lajos Markó, Gergö Attila Molnár, Zoltán Wagner, Katalin Böddi, Tamas Koszegi, Zoltan Szabó, Zoltan Matus, Istvan Szijrtó, Äkos Mérei, Géza Nagy, Istvan Wittmann

Research output: Article

4 Citations (Scopus)

Abstract

The measurement of the excretion of urinary albumin (albuminuria) is an important and well-established method to assess clinical outcomes. A high-performance liquid chromatography (HPLC) method has been introduced to measure albuminuria. Using this method, it was found that commonly used immunological methods do not measure a fraction of urinary albumin. Some authors presumed that the reason of immuno-unreactivity is the modification of urinary albumin; some others presumed that the difference is merely because of interference. In order to decide this question, we established an HPLC method equipped with tandem UV and fluorescent detection to assess the changes in the detectability of albumin with the rate of modification. For this measurement, differently modified forms of albumin were used. Urine samples of diabetic patients were also measured to find a potential connection between the modification rate and clinical parameters. Secondly, we have established a reversed phase HPLC method to assess the interference rate. We conclude that albumin modification does not affect immunoreactivity. The modification rate of urinary albumin in diabetic patients showed a correlation with renal function. The interference rate of the albumin peak was found to be 12.7% on average, which does not explain the difference between the two methods.

Original languageEnglish
Pages (from-to)1137-1150
Number of pages14
JournalPhysiological measurement
Volume30
Issue number10
DOIs
Publication statusPublished - jan. 1 2009

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Biomedical Engineering
  • Physiology (medical)

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