Matrigel-induced acinar differentiation is followed by apoptosis in HSG cells

Vanda Szlávik, János Vág, Károly Markó, Kornél Demeter, Emília Madarász, Imre Oláh, Tivadar Zelles, Brian C. O'Connell, Gábor Varga

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Abstract

It has been shown that a human salivary gland cell line (HSG) is capable of differentiation into gland-like structures, though little is known of how morphological features are formed or controlled. Here we investigated the changes in cell proliferation and apoptosis upon terminal differentiation of HSG cells in Matrigel, an extracellular matrix derivative. Changes in the expression of survivin, a prominent anti-apoptotic factor, and caspase-3, a key apoptotic factor were also measured. In order to better understand the involvement of key signal transduction pathways in this system we pharmacologically blocked the activity of tyrosine kinase, nuclear factor kappa B(NFκB), protein kinase C (PKC), phosphatidylinositol 3-kinase (PI3K) and matrix metalloproteases (MMP). Results of these studies demonstrate that cytodifferentiation of HSG cells to an acinar phenotype is accompanied first by a decrease of cell prol iteration and then by a massive programmed cell death, affected by multiple signal transduction pathways. Thus, Matrigel alone is insufficient for the full maturation and long term survival of the newly formed acini: the presence of other factors is necessary to complete the acinar differentiation of HSG cells.

Original languageEnglish
Pages (from-to)284-295
Number of pages12
JournalJournal of cellular biochemistry
Volume103
Issue number1
DOIs
Publication statusPublished - jan. 1 2008

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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