Many spinal cord cells transiently express low molecular weight forms of glutamic acid decarboxylase during embryonic development

Toby Behar, Anne Schaffner, Peter Laing, Lynn Hudson, Samuel Komoly, Jeffery Barker

Research output: Article

48 Citations (Scopus)

Abstract

At early developmental stages in the rat spinal cord (embryonic day 13), when neuronal progenitors are still proliferating, most differentiating neurons express truncated forms of glutamic acid decarboxylase (GAD) (approximately 25 kDa) which are the products of alternative splicing of the GAD67 gene. These truncated proteins do not appear to synthesize γ-aminobutyric acid (GABA). The amino acid is detected in cells only after alternative splicing of the GAD67 gene generates a full-length, 67 kDa enzymatically active form of GAD. Both the 67 kDa GAD and GABA colocalize and appear diffusely distributed in the cytoplasm of embryonic neurons. GABA does not appear associated with synaptic vesicles until after birth, when its intracellular distribution becomes punctate and it colocalizes with synaptophysin. At this time, it also colocalizes with an immunologically distinct 65 kDa GAD protein encoded by a second GAD gene (GAD65). Expression of different GAD-related proteins with distinct intracellular distributions during development suggests that GABA, the product of these enzymes, may have trophic or metabolic roles during spinal cord differentiation.

Original languageEnglish
Pages (from-to)203-218
Number of pages16
JournalDevelopmental Brain Research
Volume72
Issue number2
DOIs
Publication statusPublished - ápr. 16 1993

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

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