Lysophosphatidylcholine is a regulator of tyrosine kinase activity and intracellular Ca2+ level in Jurkat T cell line

Ádám Légrádi, Violeta Chitu, Valéria Szukacsov, R. Fajka-Boja, Kinga Székely Szücs, E. Monostori

Research output: Article

25 Citations (Scopus)

Abstract

Lysophospholipids, particularly lysophosphatidylcholine (lyso-PC), have been implicated in modulating T cell functions at the sites of inflammation and atherosclerosis. Although the chemotactic and immunomodulatory effects are well documented, the exact signaling pathway of lyso-PC action is poorly defined. In this work, we studied the earliest biochemical events in T cells triggered by lyso-PC. A marked and immediate tyrosine phosphorylation was induced in the leukemic T cell line, Jurkat. Phosphorylation of cellular substrates included src family kinase, p56lck and syk family kinase, ZAP70. The lyso-PC induced tyrosine phosphorylation was largely dependent on the presence of functional p56lck. Tyrosine phosphorylation was followed by the elevation of intracellular Ca2+ concentration. The magnitude of the mobilization of the intracellular Ca2+ was similar in the absence of the p56lck activity in JCaM1.6 cells as in Jurkat cells, however, it was slightly but reproducibly delayed compared to that in the wild type cells. Inhibition of the Ser/Thr kinases and tyrosine kinases with staurosporine and genistein, respectively, decreased the rise in the intracellular Ca 2+ content. Moreover, pertussis toxin completely blocked the Ca 2+ signal supporting the role of the G-protein coupled LPC receptor in this event.

Original languageEnglish
Pages (from-to)17-21
Number of pages5
JournalImmunology Letters
Volume91
Issue number1
DOIs
Publication statusPublished - jan. 30 2004

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Lysophosphatidylcholines
Jurkat Cells
Protein-Tyrosine Kinases
Phosphorylation
T-Lymphocytes
Tyrosine
Cell Line
Lysophospholipids
Staurosporine
src-Family Kinases
Genistein
Pertussis Toxin
G-Protein-Coupled Receptors
Atherosclerosis
Phosphotransferases
Inflammation

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Lysophosphatidylcholine is a regulator of tyrosine kinase activity and intracellular Ca2+ level in Jurkat T cell line. / Légrádi, Ádám; Chitu, Violeta; Szukacsov, Valéria; Fajka-Boja, R.; Szücs, Kinga Székely; Monostori, E.

In: Immunology Letters, Vol. 91, No. 1, 30.01.2004, p. 17-21.

Research output: Article

Légrádi, Ádám ; Chitu, Violeta ; Szukacsov, Valéria ; Fajka-Boja, R. ; Szücs, Kinga Székely ; Monostori, E. / Lysophosphatidylcholine is a regulator of tyrosine kinase activity and intracellular Ca2+ level in Jurkat T cell line. In: Immunology Letters. 2004 ; Vol. 91, No. 1. pp. 17-21.
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