Lymphocytic Infiltrates in the Spinal Cord in Amyotrophic Lateral Sclerosis

J. Engelhardt, J. Tajti, Stanley H. Appel

Research output: Article

194 Citations (Scopus)

Abstract

Immunohistochemical examination was undertaken to assess the presence of lymphocytes and lymphocyte subsets in the spinal cord in amyotrophic lateral sclerosis (ALS). —Twenty-seven consecutive ALS autopsy cases and 11 consecutive disease—control autopsy cases were examined. Tissue sections were reacted with mouse monoclonal antibody to human leukocyte common antigen, or monoclonal antibody to human B-cell L26 antigen, and detected with immunoperoxidase techniques. Unfixed sections were reacted with antihuman Leu-3a and Leu-3b/CD4 or antihuman Leu-2a/CD8 mouse monoclonal antibody and then detected with peroxidase techniques. —Tertiary care hospital. —Amyotrophic lateral sclerosis and non-ALS control autopsy specimens. —Detection of lymphocytes by histological and immunohistochemical reactivity. —Perivascular and intraparenchymal lymphocytic infiltrates were found in the spinal cord of 18 of 27 consecutive ALS autopsy cases. The lymphocytes possessed only T-cell markers; no B-cell markers could be demonstrated. T-helper cells were found in proximity to degenerating corticospinal tracts, while T-helper and T-suppressor/cytotoxic cells were demonstrated in ventral horns. Lymphocytes were present in the spinal cord of only one control specimen (multiple sclerosis) and in none of the remaining 10 control specimens. In ALS tissue, the lymphocytic infiltrates did not correlate with the rate of progression or stage of the disease or with the presence or absence of terminal infections. —T-cell lymphocytes are present in the spinal cord of patients with ALS. T-helper cells are found in proximity to corticospinal tract degeneration, while T-helper and T-suppressor/cytotoxic cells are present in ventral horns. The role of these lymphocytes remains to be elucidated.

Original languageEnglish
Pages (from-to)30-36
Number of pages7
JournalArchives of Neurology
Volume50
Issue number1
DOIs
Publication statusPublished - 1993

Fingerprint

Amyotrophic Lateral Sclerosis
Spinal Cord
Lymphocytes
Autopsy
Pyramidal Tracts
Monoclonal Antibodies
Horns
Helper-Inducer T-Lymphocytes
B-Lymphocytes
CD45 Antigens
T-Lymphocytes
Motor Neuron Disease
Lymphocyte Subsets
Tertiary Healthcare
HLA Antigens
Immunoenzyme Techniques
Tertiary Care Centers
Peroxidase
Multiple Sclerosis
Cord

ASJC Scopus subject areas

  • Arts and Humanities (miscellaneous)
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Lymphocytic Infiltrates in the Spinal Cord in Amyotrophic Lateral Sclerosis. / Engelhardt, J.; Tajti, J.; Appel, Stanley H.

In: Archives of Neurology, Vol. 50, No. 1, 1993, p. 30-36.

Research output: Article

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