Lymphocyte subset reconstitution after allogeneic bone marrow transplantation using radiation-free conditioning regimen for patients with chronic granulocytic leukemia

János Milosevits, Róbert Dénes, Anna Poros, Péter Reményi, Árpád Bátai, Anikó Barta, T. Masszi, Lilla Lengyel, Katalin Jakab, János Földi, G. Petrányi, Endre Kelemen, K. Pálóczi

Research output: Article

6 Citations (Scopus)

Abstract

Conditioning regimens for BMT are important in determining transplant outcome. A radiation-free protocol containing Mitobronitol (DBM), Cytarabine (Ara-C) and Cyclophosphamide (Cy) was used for conditioning of patients with chronic granulocytic leukemia (CGL). Using this conditioning treatment, fewer transplant related complications, including acute GVHD, VOD and severe infections, were observed. Acute GVHD did not develop, but chronic GVHD, accompanied with graft-versus leukemia, was present in half of the cases. To determine the clinical effect of the DBM/Ara-C/Cy conditioning, the recovery of peripheral blood lymphocytes was examined after allogeneic BMT for patients with CGL in comparison with TBI/Cy conditioning. The lymphocyte subsets of 11 DBM patients were followed and analyzed periodically (30-90 days, 4-12 months and > 13 months) using ten monoclonal antibodies and flow cytometry. Decreased percentage of total T cells as well as CD4+ and CD8+ subpopulations, significantly decreased T cell activation and increased proportion of TCRγδ+ cells were found to be characteristic in the early post-transplant period in the DBM group. Early recovery and consistently higher percentage of B cells were observed for the whole follow-up period of patients receiving DBM conditioning. A high proportion of NK cells was observed in all transplant recipients. These findings suggest that the characteristic pattern of recovering lymphocytes is associated with the lack of severe transplant-related clinical complications following DBM/Ara-C/Cy conditioning.

Original languageEnglish
Pages (from-to)27-33
Number of pages7
JournalInternational Journal of Hematology
Volume62
Issue number1
Publication statusPublished - júl. 1995

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Homologous Transplantation
Lymphocyte Subsets
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Bone Marrow Transplantation
Cytarabine
Cyclophosphamide
Radiation
Transplants
Mitobronitol
Lymphocytes
T-Lymphocytes
Natural Killer Cells
Flow Cytometry
Leukemia
B-Lymphocytes
Monoclonal Antibodies
Infection

ASJC Scopus subject areas

  • Hematology

Cite this

Lymphocyte subset reconstitution after allogeneic bone marrow transplantation using radiation-free conditioning regimen for patients with chronic granulocytic leukemia. / Milosevits, János; Dénes, Róbert; Poros, Anna; Reményi, Péter; Bátai, Árpád; Barta, Anikó; Masszi, T.; Lengyel, Lilla; Jakab, Katalin; Földi, János; Petrányi, G.; Kelemen, Endre; Pálóczi, K.

In: International Journal of Hematology, Vol. 62, No. 1, 07.1995, p. 27-33.

Research output: Article

Milosevits, János ; Dénes, Róbert ; Poros, Anna ; Reményi, Péter ; Bátai, Árpád ; Barta, Anikó ; Masszi, T. ; Lengyel, Lilla ; Jakab, Katalin ; Földi, János ; Petrányi, G. ; Kelemen, Endre ; Pálóczi, K. / Lymphocyte subset reconstitution after allogeneic bone marrow transplantation using radiation-free conditioning regimen for patients with chronic granulocytic leukemia. In: International Journal of Hematology. 1995 ; Vol. 62, No. 1. pp. 27-33.
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AU - Dénes, Róbert

AU - Poros, Anna

AU - Reményi, Péter

AU - Bátai, Árpád

AU - Barta, Anikó

AU - Masszi, T.

AU - Lengyel, Lilla

AU - Jakab, Katalin

AU - Földi, János

AU - Petrányi, G.

AU - Kelemen, Endre

AU - Pálóczi, K.

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