Lung volumes and respiratory mechanics in elastase-induced emphysema in mice

Z. Hantos, A. Adamicza, T. Z. Jánosi, M. V. Szabari, J. Tolnai, B. Suki

Research output: Article

35 Citations (Scopus)

Abstract

Absolute lung volumes such as functional residual capacity, residual volume (RV), and total lung capacity (TLC) are used to characterize emphysema in patients, whereas in animal models of emphysema, the mechanical parameters are invariably obtained as a function of transrespiratory pressure (Prs). The aim of the present study was to establish a link between the mechanical parameters including tissue elastance (H) and airway resistance (Raw), and thoracic gas volume (TGV) in addition to Prs in a mouse model of emphysema. Using low-frequency forced oscillations during slow deep inflation, we tracked H and Raw as functions of TGV and Prs in normal mice and mice treated with porcine pancreatic elastase. The presence of emphysema was confirmed by morphometric analysis of histological slices. The treatment resulted in an increase in TGV by 51 and 44% and a decrease in H by 57 and 27%, respectively, at 0 and 20 cmH2O of Prs. The Raw did not differ between the groups at any value of Prs, but it was significantly higher in the treated mice at comparable TGV values. In further groups of mice, tracheal sounds were recorded during inflations from RV to TLC. All lung volumes but RV were significantly elevated in the treated mice, whereas the numbers and size distributions of inspiratory crackles were not different, suggesting that the airways were not affected by the elastase treatment. These findings emphasize the importance of absolute lung volumes and indicate that tissue destruction was not associated with airway dysfunction in this mouse model of emphysema.

Original languageEnglish
Pages (from-to)1864-1872
Number of pages9
JournalJournal of Applied Physiology
Volume105
Issue number6
DOIs
Publication statusPublished - dec. 2008

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Respiratory Mechanics
Pancreatic Elastase
Emphysema
Lung
Residual Volume
Pressure
Thorax
Gases
Total Lung Capacity
Economic Inflation
Functional Residual Capacity
Airway Resistance
Respiratory Sounds
Swine
Animal Models
Therapeutics

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Lung volumes and respiratory mechanics in elastase-induced emphysema in mice. / Hantos, Z.; Adamicza, A.; Jánosi, T. Z.; Szabari, M. V.; Tolnai, J.; Suki, B.

In: Journal of Applied Physiology, Vol. 105, No. 6, 12.2008, p. 1864-1872.

Research output: Article

Hantos, Z. ; Adamicza, A. ; Jánosi, T. Z. ; Szabari, M. V. ; Tolnai, J. ; Suki, B. / Lung volumes and respiratory mechanics in elastase-induced emphysema in mice. In: Journal of Applied Physiology. 2008 ; Vol. 105, No. 6. pp. 1864-1872.
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