Low temperature inhibits RNA silencing-mediated defence by the control of siRNA generation

Research output: Article

316 Citations (Scopus)

Abstract

Temperature dramatically affects plant-virus interactions. Outbreaks of virus diseases are frequently associated with low temperature, while at high temperature viral symptoms are often attenuated (heat masking) and plants rapidly recover from virus diseases. However, the underlying mechanisms of these well-known observations are not yet understood. RNA silencing is a conserved defence system of eukaryotic cells, which operates against molecular parasites including viruses and transgenes. Here we show that at low temperature both virus and transgene triggered RNA silencing are inhibited. Therefore, in cold, plants become more susceptible to viruses, and RNA silencing-based phenotypes of transgenic plants are lost. Consistently, the levels of virus- and transgene-derived small (21-26 nucleotide) interfering (si) RNAs - the central molecules of RNA silencing-mediated defence pathways - are dramatically reduced at low temperature. In contrast, RNA silencing was activated and the amount of siRNAs gradually increased with rising temperature. However, temperature does not influence the accumulation of micro (mi) RNAs, which play a role in developmental regulation, suggesting that the two classes of small (si and mi) RNAs are generated by different nuclease complexes.

Original languageEnglish
Pages (from-to)633-640
Number of pages8
JournalEMBO Journal
Volume22
Issue number3
DOIs
Publication statusPublished - febr. 3 2003

Fingerprint

RNA Interference
Viruses
Small Interfering RNA
RNA
Temperature
Transgenes
Virus Diseases
MicroRNAs
Plant Viruses
Genetically Modified Plants
Eukaryotic Cells
Disease Outbreaks
Parasites
Nucleotides
Hot Temperature
Phenotype
Molecules

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

@article{eb993525a84847fcb9747b0e45169957,
title = "Low temperature inhibits RNA silencing-mediated defence by the control of siRNA generation",
abstract = "Temperature dramatically affects plant-virus interactions. Outbreaks of virus diseases are frequently associated with low temperature, while at high temperature viral symptoms are often attenuated (heat masking) and plants rapidly recover from virus diseases. However, the underlying mechanisms of these well-known observations are not yet understood. RNA silencing is a conserved defence system of eukaryotic cells, which operates against molecular parasites including viruses and transgenes. Here we show that at low temperature both virus and transgene triggered RNA silencing are inhibited. Therefore, in cold, plants become more susceptible to viruses, and RNA silencing-based phenotypes of transgenic plants are lost. Consistently, the levels of virus- and transgene-derived small (21-26 nucleotide) interfering (si) RNAs - the central molecules of RNA silencing-mediated defence pathways - are dramatically reduced at low temperature. In contrast, RNA silencing was activated and the amount of siRNAs gradually increased with rising temperature. However, temperature does not influence the accumulation of micro (mi) RNAs, which play a role in developmental regulation, suggesting that the two classes of small (si and mi) RNAs are generated by different nuclease complexes.",
keywords = "Heat masking, MiRNA, RNA silencing, SiRNA, Temperature dependence",
author = "Gy{\"o}rgy Szittya and D{\'a}niel Silhavy and Attila Moln{\'a}r and Zolt{\'a}n Havelda and {\'A}gnes Lovas and L{\'o}r{\'a}nt Lakatos and Zs{\'o}fia B{\'a}nfalvi and J{\'o}zsef Burgy{\'a}n",
year = "2003",
month = "2",
day = "3",
doi = "10.1093/emboj/cdg74",
language = "English",
volume = "22",
pages = "633--640",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "3",

}

TY - JOUR

T1 - Low temperature inhibits RNA silencing-mediated defence by the control of siRNA generation

AU - Szittya, György

AU - Silhavy, Dániel

AU - Molnár, Attila

AU - Havelda, Zoltán

AU - Lovas, Ágnes

AU - Lakatos, Lóránt

AU - Bánfalvi, Zsófia

AU - Burgyán, József

PY - 2003/2/3

Y1 - 2003/2/3

N2 - Temperature dramatically affects plant-virus interactions. Outbreaks of virus diseases are frequently associated with low temperature, while at high temperature viral symptoms are often attenuated (heat masking) and plants rapidly recover from virus diseases. However, the underlying mechanisms of these well-known observations are not yet understood. RNA silencing is a conserved defence system of eukaryotic cells, which operates against molecular parasites including viruses and transgenes. Here we show that at low temperature both virus and transgene triggered RNA silencing are inhibited. Therefore, in cold, plants become more susceptible to viruses, and RNA silencing-based phenotypes of transgenic plants are lost. Consistently, the levels of virus- and transgene-derived small (21-26 nucleotide) interfering (si) RNAs - the central molecules of RNA silencing-mediated defence pathways - are dramatically reduced at low temperature. In contrast, RNA silencing was activated and the amount of siRNAs gradually increased with rising temperature. However, temperature does not influence the accumulation of micro (mi) RNAs, which play a role in developmental regulation, suggesting that the two classes of small (si and mi) RNAs are generated by different nuclease complexes.

AB - Temperature dramatically affects plant-virus interactions. Outbreaks of virus diseases are frequently associated with low temperature, while at high temperature viral symptoms are often attenuated (heat masking) and plants rapidly recover from virus diseases. However, the underlying mechanisms of these well-known observations are not yet understood. RNA silencing is a conserved defence system of eukaryotic cells, which operates against molecular parasites including viruses and transgenes. Here we show that at low temperature both virus and transgene triggered RNA silencing are inhibited. Therefore, in cold, plants become more susceptible to viruses, and RNA silencing-based phenotypes of transgenic plants are lost. Consistently, the levels of virus- and transgene-derived small (21-26 nucleotide) interfering (si) RNAs - the central molecules of RNA silencing-mediated defence pathways - are dramatically reduced at low temperature. In contrast, RNA silencing was activated and the amount of siRNAs gradually increased with rising temperature. However, temperature does not influence the accumulation of micro (mi) RNAs, which play a role in developmental regulation, suggesting that the two classes of small (si and mi) RNAs are generated by different nuclease complexes.

KW - Heat masking

KW - MiRNA

KW - RNA silencing

KW - SiRNA

KW - Temperature dependence

UR - http://www.scopus.com/inward/record.url?scp=0037415726&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037415726&partnerID=8YFLogxK

U2 - 10.1093/emboj/cdg74

DO - 10.1093/emboj/cdg74

M3 - Article

C2 - 12554663

AN - SCOPUS:0037415726

VL - 22

SP - 633

EP - 640

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 3

ER -