Low-temperature-induced accumulation of xanthophylls and its structural consequences in the photosynthetic membranes of the cyanobacterium Cylindrospermopsis raciborskii: An FTIR spectroscopic study

Z. Várkonyi, Kazuomori Masamoto, Mónika Debreczeny, Ottó Zsiros, Bettina Ughy, Z. Gombos, Ildikó Domonkos, T. Farkas, Hajime Wada, B. Szalontai

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Abstract

The effects of the growth temperature on the lipids and carotenoids of a filamentous cyanobacterium, Cylindrospermopsis raciborskii, were studied., The relative amounts of polyunsaturated glycerolipids and myxoxanthophylls in the thylakoid membranes increased markedly when this cyanobacterium was grown at 25°C instead of 35°C. Fourier transform infrared spectroscopy was used to analyze the low-temperature-induced structural alterations in the thylakoid membranes. Despite the higher amount of unsaturated lipids there, conventional analysis of the vsymCH2 band (characteristic of the lipid disorder) revealed more tightly arranged fatty-acyl chains for the thylakoids in the cells grown at 25°C as compared with those grown at 35°C. This apparent controversy was resolved by a two-component analysis of the vsymCH2 band, which demonstrated very rigid, myxoxanthophyll-related lipids in the thylakoid membranes. When this rigid component was excluded from the analysis of the thermotropic responses of the vsymCH2 bands, the expected higher fatty-acyl disorder was observed for the thylakoids prepared from cells grown at 25°C as compared with those grown at 35°C. Both the carotenoid composition and this rigid component in the thylakoid membranes were only growth temperature-dependent; the intensity of the illuminating light during cultivation had no apparent effect on these parameters. We propose that, besides their well-known protective functions, the polar carotenoids in particular may have structural effects on the thylakoid membranes. These effects should be exerted locally-by forming protective patches, in-membrane barriers of low dynamics-to prevent the access of reactive radicals generated in either enzymatic or photosynthetic processes to sensitive spots of the membranes.

Original languageEnglish
Pages (from-to)2410-2415
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number4
DOIs
Publication statusPublished - febr. 19 2002

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Cylindrospermopsis
Xanthophylls
Thylakoids
Cyanobacteria
Fourier Transform Infrared Spectroscopy
Temperature
Membranes
Carotenoids
Lipids
Growth
Light

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

@article{36fe9c9f08d44950abc1016571baf2e9,
title = "Low-temperature-induced accumulation of xanthophylls and its structural consequences in the photosynthetic membranes of the cyanobacterium Cylindrospermopsis raciborskii: An FTIR spectroscopic study",
abstract = "The effects of the growth temperature on the lipids and carotenoids of a filamentous cyanobacterium, Cylindrospermopsis raciborskii, were studied., The relative amounts of polyunsaturated glycerolipids and myxoxanthophylls in the thylakoid membranes increased markedly when this cyanobacterium was grown at 25°C instead of 35°C. Fourier transform infrared spectroscopy was used to analyze the low-temperature-induced structural alterations in the thylakoid membranes. Despite the higher amount of unsaturated lipids there, conventional analysis of the vsymCH2 band (characteristic of the lipid disorder) revealed more tightly arranged fatty-acyl chains for the thylakoids in the cells grown at 25°C as compared with those grown at 35°C. This apparent controversy was resolved by a two-component analysis of the vsymCH2 band, which demonstrated very rigid, myxoxanthophyll-related lipids in the thylakoid membranes. When this rigid component was excluded from the analysis of the thermotropic responses of the vsymCH2 bands, the expected higher fatty-acyl disorder was observed for the thylakoids prepared from cells grown at 25°C as compared with those grown at 35°C. Both the carotenoid composition and this rigid component in the thylakoid membranes were only growth temperature-dependent; the intensity of the illuminating light during cultivation had no apparent effect on these parameters. We propose that, besides their well-known protective functions, the polar carotenoids in particular may have structural effects on the thylakoid membranes. These effects should be exerted locally-by forming protective patches, in-membrane barriers of low dynamics-to prevent the access of reactive radicals generated in either enzymatic or photosynthetic processes to sensitive spots of the membranes.",
author = "Z. V{\'a}rkonyi and Kazuomori Masamoto and M{\'o}nika Debreczeny and Ott{\'o} Zsiros and Bettina Ughy and Z. Gombos and Ildik{\'o} Domonkos and T. Farkas and Hajime Wada and B. Szalontai",
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month = "2",
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language = "English",
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T1 - Low-temperature-induced accumulation of xanthophylls and its structural consequences in the photosynthetic membranes of the cyanobacterium Cylindrospermopsis raciborskii

T2 - An FTIR spectroscopic study

AU - Várkonyi, Z.

AU - Masamoto, Kazuomori

AU - Debreczeny, Mónika

AU - Zsiros, Ottó

AU - Ughy, Bettina

AU - Gombos, Z.

AU - Domonkos, Ildikó

AU - Farkas, T.

AU - Wada, Hajime

AU - Szalontai, B.

PY - 2002/2/19

Y1 - 2002/2/19

N2 - The effects of the growth temperature on the lipids and carotenoids of a filamentous cyanobacterium, Cylindrospermopsis raciborskii, were studied., The relative amounts of polyunsaturated glycerolipids and myxoxanthophylls in the thylakoid membranes increased markedly when this cyanobacterium was grown at 25°C instead of 35°C. Fourier transform infrared spectroscopy was used to analyze the low-temperature-induced structural alterations in the thylakoid membranes. Despite the higher amount of unsaturated lipids there, conventional analysis of the vsymCH2 band (characteristic of the lipid disorder) revealed more tightly arranged fatty-acyl chains for the thylakoids in the cells grown at 25°C as compared with those grown at 35°C. This apparent controversy was resolved by a two-component analysis of the vsymCH2 band, which demonstrated very rigid, myxoxanthophyll-related lipids in the thylakoid membranes. When this rigid component was excluded from the analysis of the thermotropic responses of the vsymCH2 bands, the expected higher fatty-acyl disorder was observed for the thylakoids prepared from cells grown at 25°C as compared with those grown at 35°C. Both the carotenoid composition and this rigid component in the thylakoid membranes were only growth temperature-dependent; the intensity of the illuminating light during cultivation had no apparent effect on these parameters. We propose that, besides their well-known protective functions, the polar carotenoids in particular may have structural effects on the thylakoid membranes. These effects should be exerted locally-by forming protective patches, in-membrane barriers of low dynamics-to prevent the access of reactive radicals generated in either enzymatic or photosynthetic processes to sensitive spots of the membranes.

AB - The effects of the growth temperature on the lipids and carotenoids of a filamentous cyanobacterium, Cylindrospermopsis raciborskii, were studied., The relative amounts of polyunsaturated glycerolipids and myxoxanthophylls in the thylakoid membranes increased markedly when this cyanobacterium was grown at 25°C instead of 35°C. Fourier transform infrared spectroscopy was used to analyze the low-temperature-induced structural alterations in the thylakoid membranes. Despite the higher amount of unsaturated lipids there, conventional analysis of the vsymCH2 band (characteristic of the lipid disorder) revealed more tightly arranged fatty-acyl chains for the thylakoids in the cells grown at 25°C as compared with those grown at 35°C. This apparent controversy was resolved by a two-component analysis of the vsymCH2 band, which demonstrated very rigid, myxoxanthophyll-related lipids in the thylakoid membranes. When this rigid component was excluded from the analysis of the thermotropic responses of the vsymCH2 bands, the expected higher fatty-acyl disorder was observed for the thylakoids prepared from cells grown at 25°C as compared with those grown at 35°C. Both the carotenoid composition and this rigid component in the thylakoid membranes were only growth temperature-dependent; the intensity of the illuminating light during cultivation had no apparent effect on these parameters. We propose that, besides their well-known protective functions, the polar carotenoids in particular may have structural effects on the thylakoid membranes. These effects should be exerted locally-by forming protective patches, in-membrane barriers of low dynamics-to prevent the access of reactive radicals generated in either enzymatic or photosynthetic processes to sensitive spots of the membranes.

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U2 - 10.1073/pnas.042698799

DO - 10.1073/pnas.042698799

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JF - Proceedings of the National Academy of Sciences of the United States of America

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