Localization and production of proteoglycans by HT1080 cell lines with altered N-ras expression

J. Tímár, H. Paterson

Research output: Article

8 Citations (Scopus)

Abstract

The alterations in the production of proteoglycans in relation to the expression of the malignant phenotype - controlled by the level of expression of activated N-ras gene - was studied in HT1080 human fibrosarcoma cells and in its revertant variants rev.1c and rev. 10a. A decreased production of radiolabelled PGs - especially HSPGs - was observed in HT1080 cells, compared to the revertant lines. By immunofluorescence, the HSPG epitopes were localized mainly into the putative endoplasmic reticulum and/or Golgi zone in HT1080 cells, and to the diffuse cytoplasmic and membrane localization in revertant lines. It is suggested, that the altered expression of PGs represents an important aspect of the transformed phenotype of HT1080 fibrosarcoma cells.

Original languageEnglish
Pages (from-to)145-150
Number of pages6
JournalCancer Letters
Volume53
Issue number2-3
DOIs
Publication statusPublished - 1990

Fingerprint

Proteoglycans
Cell Line
Heparan Sulfate Proteoglycans
Fibrosarcoma
Phenotype
ras Genes
Endoplasmic Reticulum
Fluorescent Antibody Technique
Epitopes
Cell Membrane

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Localization and production of proteoglycans by HT1080 cell lines with altered N-ras expression. / Tímár, J.; Paterson, H.

In: Cancer Letters, Vol. 53, No. 2-3, 1990, p. 145-150.

Research output: Article

@article{bf0bf4c7516048199ac46252c6227b16,
title = "Localization and production of proteoglycans by HT1080 cell lines with altered N-ras expression",
abstract = "The alterations in the production of proteoglycans in relation to the expression of the malignant phenotype - controlled by the level of expression of activated N-ras gene - was studied in HT1080 human fibrosarcoma cells and in its revertant variants rev.1c and rev. 10a. A decreased production of radiolabelled PGs - especially HSPGs - was observed in HT1080 cells, compared to the revertant lines. By immunofluorescence, the HSPG epitopes were localized mainly into the putative endoplasmic reticulum and/or Golgi zone in HT1080 cells, and to the diffuse cytoplasmic and membrane localization in revertant lines. It is suggested, that the altered expression of PGs represents an important aspect of the transformed phenotype of HT1080 fibrosarcoma cells.",
keywords = "human fibrosarcoma cells, N-ras, proteoglycans",
author = "J. T{\'i}m{\'a}r and H. Paterson",
year = "1990",
doi = "10.1016/0304-3835(90)90207-E",
language = "English",
volume = "53",
pages = "145--150",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "2-3",

}

TY - JOUR

T1 - Localization and production of proteoglycans by HT1080 cell lines with altered N-ras expression

AU - Tímár, J.

AU - Paterson, H.

PY - 1990

Y1 - 1990

N2 - The alterations in the production of proteoglycans in relation to the expression of the malignant phenotype - controlled by the level of expression of activated N-ras gene - was studied in HT1080 human fibrosarcoma cells and in its revertant variants rev.1c and rev. 10a. A decreased production of radiolabelled PGs - especially HSPGs - was observed in HT1080 cells, compared to the revertant lines. By immunofluorescence, the HSPG epitopes were localized mainly into the putative endoplasmic reticulum and/or Golgi zone in HT1080 cells, and to the diffuse cytoplasmic and membrane localization in revertant lines. It is suggested, that the altered expression of PGs represents an important aspect of the transformed phenotype of HT1080 fibrosarcoma cells.

AB - The alterations in the production of proteoglycans in relation to the expression of the malignant phenotype - controlled by the level of expression of activated N-ras gene - was studied in HT1080 human fibrosarcoma cells and in its revertant variants rev.1c and rev. 10a. A decreased production of radiolabelled PGs - especially HSPGs - was observed in HT1080 cells, compared to the revertant lines. By immunofluorescence, the HSPG epitopes were localized mainly into the putative endoplasmic reticulum and/or Golgi zone in HT1080 cells, and to the diffuse cytoplasmic and membrane localization in revertant lines. It is suggested, that the altered expression of PGs represents an important aspect of the transformed phenotype of HT1080 fibrosarcoma cells.

KW - human fibrosarcoma cells

KW - N-ras

KW - proteoglycans

UR - http://www.scopus.com/inward/record.url?scp=0025113713&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025113713&partnerID=8YFLogxK

U2 - 10.1016/0304-3835(90)90207-E

DO - 10.1016/0304-3835(90)90207-E

M3 - Article

C2 - 2208074

AN - SCOPUS:0025113713

VL - 53

SP - 145

EP - 150

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 2-3

ER -