Liquid chromatographic and mass spectrometric analysis of human serum acid alpha-1-glycoprotein

T. Kremmer, É Szöllösi, M. Boldizsár, B. Vincze, K. Ludányi, T. Imre, G. Schlosser, K. Vékey

Research output: Article

31 Citations (Scopus)

Abstract

Human serum acid alpha-1-glycoprotein (AGP, orosomucoid) content of healthy individuals and cancer patients was measured, isolated and purified using a protocol of fast and biocompatible sample preparation, ion exchange and dye-ligand affinity chromatographic methods. In comparison to the healthy individuals significantly higher serum AGP levels were found in a wide spectrum of cancer patients, indicating its diagnostic value in the malignant disease. Oligosaccharide content of AGP samples was separated following PNGase F enzyme digestion and analysed by RP-HPLC and MALDI-TOF mass spectrometry. RP-HPLC and MALDI-TOF mass spectrometric analysis of sugar constituents of AGP specimen originated from selected cancer patients with high serum AGP levels indicated the appearance of anomal distribution of bi-, tri- and tetra-antennary oligosaccharide structures compared to the healthy controls.

Original languageEnglish
Pages (from-to)323-329
Number of pages7
JournalBiomedical Chromatography
Volume18
Issue number5
DOIs
Publication statusPublished - jún. 1 2004

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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