Light and Temperature Signalling at the Level of CBF14 Gene Expression in Wheat and Barley

Aliz Novák, Boldizsár, Krisztián Gierczik, Attila Vágújfalvi, E. Ádám, L. Kozma-Bognár, G. Galiba

Research output: Article

3 Citations (Scopus)

Abstract

The wheat and barley CBF14 genes have been newly defined as key components of the light quality-dependent regulation of the freezing tolerance by the integration of phytochrome-mediated light and temperature signals. To further investigate the wavelength dependence of light-induced CBF14 expression in cereals, we carried out a detailed study using monochromatic light treatments at an inductive and a non-inductive temperature. Transcript levels of CBF14 gene in winter wheat Cheyenne, winter einkorn G3116 and winter barley Nure genotypes were monitored. We demonstrated that (1) CBF14 is most effectively induced by blue light and (2) provide evidence that this induction does not arise from light-controlled CRY gene expression. (3) We demonstrate that temperature shifts induce CBF14 transcription independent of the light conditions and that (4) the effect of temperature and light treatments are additive. Based on these data, it can be assumed that temperature and light signals are relayed to the level of CBF14 expression via separate signalling routes.

Original languageEnglish
Pages (from-to)399-408
Number of pages10
JournalPlant Molecular Biology Reporter
Volume35
Issue number4
DOIs
Publication statusPublished - aug. 1 2017

Fingerprint

Hordeum
Triticum
barley
Gene Expression
Light
gene expression
wheat
Temperature
temperature
Triticum monococcum subsp. monococcum
winter barley
light quality
Phytochrome
phytochrome
blue light
cold tolerance
winter wheat
wavelengths
genes
transcription (genetics)

Keywords

    ASJC Scopus subject areas

    • Molecular Biology
    • Plant Science

    Cite this

    Light and Temperature Signalling at the Level of CBF14 Gene Expression in Wheat and Barley. / Novák, Aliz; Boldizsár; Gierczik, Krisztián; Vágújfalvi, Attila; Ádám, E.; Kozma-Bognár, L.; Galiba, G.

    In: Plant Molecular Biology Reporter, Vol. 35, No. 4, 01.08.2017, p. 399-408.

    Research output: Article

    Novák, Aliz ; Boldizsár ; Gierczik, Krisztián ; Vágújfalvi, Attila ; Ádám, E. ; Kozma-Bognár, L. ; Galiba, G. / Light and Temperature Signalling at the Level of CBF14 Gene Expression in Wheat and Barley. In: Plant Molecular Biology Reporter. 2017 ; Vol. 35, No. 4. pp. 399-408.
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