Layer chromatography-bioassays directed screening and identification of antibacterial compounds from Scotch thistle

A. Móricz, Dániel Krüzselyi, Ágnes Alberti, András Darcsi, G. Horváth, P. Csontos, S. Béni, P. Ott

Research output: Article

2 Citations (Scopus)

Abstract

The antibacterial profiling of Onopordum acanthium L. leaf extract and subsequent targeted identification of active compounds is demonstrated. Thin-layer chromatography (TLC) and off-line overpressured layer chromatography (OPLC) coupled with direct bioautography were utilized for investigation of the extract against eight bacterial strains including two plant and three human pathogens and a soil, a marine and a probiotic human gut bacteria. Antibacterial fractions obtaining infusion-transfusion OPLC were transferred to HPLC–MS/MS analysis that resulted in the characterization of three active compounds and two of them were identified as, linoleic and linolenic acid. OPLC method was adopted to preparative-scale flash chromatography for the isolation of the third active compound, which was identified after a further semi-preparative HPLC purification as the germacranolide sesquiterpene lactone onopordopicrin. Pure onopordopicrin exhibited antibacterial activity that was specified as minimal inhibitory concentration in the liquid phase as well.

Original languageEnglish
Pages (from-to)266-272
Number of pages7
JournalJournal of Chromatography A
Volume1524
DOIs
Publication statusPublished - nov. 17 2017

Fingerprint

Bioassay
Chromatography
Biological Assay
Screening
Onopordum
Thin layer chromatography
alpha-Linolenic Acid
Sesquiterpenes
Probiotics
Lactones
Pathogens
Linoleic Acid
Thin Layer Chromatography
Purification
Bacteria
Soil
High Pressure Liquid Chromatography
Soils
Liquids
onopordopicrin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

@article{ce2fedbfe4754af7872252979c58b12f,
title = "Layer chromatography-bioassays directed screening and identification of antibacterial compounds from Scotch thistle",
abstract = "The antibacterial profiling of Onopordum acanthium L. leaf extract and subsequent targeted identification of active compounds is demonstrated. Thin-layer chromatography (TLC) and off-line overpressured layer chromatography (OPLC) coupled with direct bioautography were utilized for investigation of the extract against eight bacterial strains including two plant and three human pathogens and a soil, a marine and a probiotic human gut bacteria. Antibacterial fractions obtaining infusion-transfusion OPLC were transferred to HPLC–MS/MS analysis that resulted in the characterization of three active compounds and two of them were identified as, linoleic and linolenic acid. OPLC method was adopted to preparative-scale flash chromatography for the isolation of the third active compound, which was identified after a further semi-preparative HPLC purification as the germacranolide sesquiterpene lactone onopordopicrin. Pure onopordopicrin exhibited antibacterial activity that was specified as minimal inhibitory concentration in the liquid phase as well.",
keywords = "Bioassay-guided isolation, Lactobacillus plantarum, Overpressured layer chromatography-direct bioautography, Overpressured layer chromatography-UV/fractionation, Thin-layer chromatography-direct bioautography",
author = "A. M{\'o}ricz and D{\'a}niel Kr{\"u}zselyi and {\'A}gnes Alberti and Andr{\'a}s Darcsi and G. Horv{\'a}th and P. Csontos and S. B{\'e}ni and P. Ott",
year = "2017",
month = "11",
day = "17",
doi = "10.1016/j.chroma.2017.09.062",
language = "English",
volume = "1524",
pages = "266--272",
journal = "Journal of Chromatography A",
issn = "0021-9673",

}

TY - JOUR

T1 - Layer chromatography-bioassays directed screening and identification of antibacterial compounds from Scotch thistle

AU - Móricz, A.

AU - Krüzselyi, Dániel

AU - Alberti, Ágnes

AU - Darcsi, András

AU - Horváth, G.

AU - Csontos, P.

AU - Béni, S.

AU - Ott, P.

PY - 2017/11/17

Y1 - 2017/11/17

N2 - The antibacterial profiling of Onopordum acanthium L. leaf extract and subsequent targeted identification of active compounds is demonstrated. Thin-layer chromatography (TLC) and off-line overpressured layer chromatography (OPLC) coupled with direct bioautography were utilized for investigation of the extract against eight bacterial strains including two plant and three human pathogens and a soil, a marine and a probiotic human gut bacteria. Antibacterial fractions obtaining infusion-transfusion OPLC were transferred to HPLC–MS/MS analysis that resulted in the characterization of three active compounds and two of them were identified as, linoleic and linolenic acid. OPLC method was adopted to preparative-scale flash chromatography for the isolation of the third active compound, which was identified after a further semi-preparative HPLC purification as the germacranolide sesquiterpene lactone onopordopicrin. Pure onopordopicrin exhibited antibacterial activity that was specified as minimal inhibitory concentration in the liquid phase as well.

AB - The antibacterial profiling of Onopordum acanthium L. leaf extract and subsequent targeted identification of active compounds is demonstrated. Thin-layer chromatography (TLC) and off-line overpressured layer chromatography (OPLC) coupled with direct bioautography were utilized for investigation of the extract against eight bacterial strains including two plant and three human pathogens and a soil, a marine and a probiotic human gut bacteria. Antibacterial fractions obtaining infusion-transfusion OPLC were transferred to HPLC–MS/MS analysis that resulted in the characterization of three active compounds and two of them were identified as, linoleic and linolenic acid. OPLC method was adopted to preparative-scale flash chromatography for the isolation of the third active compound, which was identified after a further semi-preparative HPLC purification as the germacranolide sesquiterpene lactone onopordopicrin. Pure onopordopicrin exhibited antibacterial activity that was specified as minimal inhibitory concentration in the liquid phase as well.

KW - Bioassay-guided isolation

KW - Lactobacillus plantarum

KW - Overpressured layer chromatography-direct bioautography

KW - Overpressured layer chromatography-UV/fractionation

KW - Thin-layer chromatography-direct bioautography

UR - http://www.scopus.com/inward/record.url?scp=85030651394&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85030651394&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2017.09.062

DO - 10.1016/j.chroma.2017.09.062

M3 - Article

VL - 1524

SP - 266

EP - 272

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

ER -