Large scale preparation of hirudin

D. Bagdy, E. Barabás, L. Gráf

Research output: Article

21 Citations (Scopus)

Abstract

A process for large scale production of hirudin from whole leeches is described. Crude hirudin is prepared by aqueous extraction, adsorption and elution on/from bentonite and subsequently some other fractionation steps. Purification of crude hirudin is carried out by chromatography on Ecteola-cellulose followed by rechromatography on CM-Sephadex C-25 and Sephadex G 75. Homogeneity of the end product was tested by end group analysis and ultracentrifugation. The process presented here can be regarded as the first large scale preparation of hirudin.

Original languageEnglish
Pages (from-to)229-238
Number of pages10
JournalThrombosis Research
Volume2
Issue number3
DOIs
Publication statusPublished - 1973

Fingerprint

Hirudins
Bentonite
Leeches
Ultracentrifugation
Cellulose
Adsorption
Chromatography
sephadex

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Hematology

Cite this

Large scale preparation of hirudin. / Bagdy, D.; Barabás, E.; Gráf, L.

In: Thrombosis Research, Vol. 2, No. 3, 1973, p. 229-238.

Research output: Article

Bagdy, D. ; Barabás, E. ; Gráf, L. / Large scale preparation of hirudin. In: Thrombosis Research. 1973 ; Vol. 2, No. 3. pp. 229-238.
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