Large-scale carbohydrate analysis by capillary array electrophoresis: Part 2. Data normalization and quantification

Julia Khandurina, Abraham A. Anderson, Nels A. Olson, Justin T. Stege, A. Guttman

Research output: Article

17 Citations (Scopus)

Abstract

Automated 96-capillary array electrophoresis (CAE) methodology described in the first part of the present work offered large-scale high-performance profiling of oligo- and monosaccharides to fulfill the needs of bioindustrial laboratories. Sensitivity at low nanomolar concentration, good resolving power and reliability achieved in the experiments is invaluable for monitoring reaction products from enzymatic polysaccharide digestion with numerous applications in agricultural, chemical and food industries. In addition to optimization of mono- and oligosaccharide separations in CAE system and necessary operational protocol modifications, capillary-to-capillary and run-to-run variation in migration time and signal intensity necessitated development of data normalization tools. Internal fluorescent standards have been incorporated into the analysis aiding migration time normalization and CAE trace alignment. Data processing, visualization, and programming tools have been developed along with quantification approaches.

Original languageEnglish
Pages (from-to)3122-3127
Number of pages6
JournalElectrophoresis
Volume25
Issue number18-19
DOIs
Publication statusPublished - okt. 2004

Fingerprint

Capillary Electrophoresis
Electrophoresis
Monosaccharides
Carbohydrates
Oligosaccharides
Agrochemicals
Chemical Industry
Food Industry
Optical resolving power
Reaction products
Polysaccharides
Digestion
Visualization
Monitoring
Industry
Experiments

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Large-scale carbohydrate analysis by capillary array electrophoresis : Part 2. Data normalization and quantification. / Khandurina, Julia; Anderson, Abraham A.; Olson, Nels A.; Stege, Justin T.; Guttman, A.

In: Electrophoresis, Vol. 25, No. 18-19, 10.2004, p. 3122-3127.

Research output: Article

Khandurina, Julia ; Anderson, Abraham A. ; Olson, Nels A. ; Stege, Justin T. ; Guttman, A. / Large-scale carbohydrate analysis by capillary array electrophoresis : Part 2. Data normalization and quantification. In: Electrophoresis. 2004 ; Vol. 25, No. 18-19. pp. 3122-3127.
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