Label-free immunosensor for monitoring vitellogenin as a biomarker for exogenous oestrogen compounds in amphibian species

Krisztina Majer-Baranyi, N. Adányi, András Nagy, Olga Bukovskaya, István Szendrő, A. Székács

Research output: Article

5 Citations (Scopus)

Abstract

A label-free, optical waveguide lightmode spectroscopy based immunosensor was developed for frog (Bombina orientalis) vitellogenin (Vtg) determination in biological samples as a biomarker for exogenous oestrogen compounds. Antibody against Vtg was produced in rabbits immunised with purified lipovitellin (Lpv), a precursor of Vtg, from the homogenised ovary of oriental fire-bellied toads (B. orientalis). The purified protein and Lpv/Vtg-specific serum were applied in both competitive and direct immunoassay formats using optical waveguide lightmode spectroscopy immunosensor. When measuring Vtg in direct manner, the Lpv antibody (1.76 µg mL−1) was immobilised on the sensor surface, and the linear measuring range for Vtg was 0.1–10 µg mL−1. During the competitive measurement, 100 ng mL−1 Lpv was applied for the immobilisation. The linear measuring range for Vtg was 0.5–50 ng mL−1. We studied the relative substrate specificity of the antibody, and it was concluded that the method is suitable for the sensitive and selective determination of Vtg levels in toads. Heart, liver and gonad samples from male animals were spiked with Vtg and were analysed using the newly developed method, and female toads and spawn samples were tested and compared to the calibration curve obtained by the spiked samples.

Original languageEnglish
Pages (from-to)481-493
Number of pages13
JournalInternational Journal of Environmental Analytical Chemistry
Volume95
Issue number6
DOIs
Publication statusPublished - máj. 3 2015

Fingerprint

Vitellogenins
Immunosensors
toad
vitellogenin
Amphibians
Biomarkers
Antibodies
amphibian
antibody
estrogens
amphibians
biomarker
Labels
biomarkers
Estrogens
Optical waveguides
Monitoring
monitoring
Anura
spectroscopy

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Soil Science
  • Health, Toxicology and Mutagenesis
  • Pollution
  • Waste Management and Disposal
  • Water Science and Technology
  • Public Health, Environmental and Occupational Health

Cite this

Label-free immunosensor for monitoring vitellogenin as a biomarker for exogenous oestrogen compounds in amphibian species. / Majer-Baranyi, Krisztina; Adányi, N.; Nagy, András; Bukovskaya, Olga; Szendrő, István; Székács, A.

In: International Journal of Environmental Analytical Chemistry, Vol. 95, No. 6, 03.05.2015, p. 481-493.

Research output: Article

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