Kin1 is a plasma membrane-associated kinase that regulates the cell surface in fission yeast

Angela Cadou, Anne Couturier, Cathy Le Goff, Teresa Soto, I. Miklós, M. Sipiczki, Linfeng Xie, James R. Paulson, Jose Cansado, Xavier Le Goff

Research output: Article

13 Citations (Scopus)

Abstract

Cell morphogenesis is a complex process that depends on cytoskeleton and membrane organization, intracellular signalling and vesicular trafficking. The rod shape of the fission yeast Schizosaccharomyces pombe and the availability of powerful genetic tools make this species an excellent model to study cell morphology. Here we have investigated the function of the conserved Kin1 kinase. Kin1-GFP associates dynamically with the plasma membrane at sites of active cell surface remodelling and is present in the membrane fraction. Kin1Δ; null cells show severe defects in cell wall structure and are unable to maintain a rod shape. To explore Kin1 primary function, we constructed an ATP analogue-sensitive allele kin1-as1. Kin1 inhibition primarily promotes delocalization of plasma membrane-associated markers of actively growing cell surface regions. Kin1 itself is depolarized and its mobility is strongly reduced. Subsequently, amorphous cell wall material accumulates at the cell surface, a phenotype that is dependent on vesicular trafficking, and the cell wall integrity mitogen-activated protein kinase pathway is activated. Deletion of cell wall integrity mitogen-activated protein kinase components reduces kin1Δ; hypersensitivity to stresses such as those induced by Calcofluor white and SDS. We propose that Kin1 is required for a tight link between the plasma membrane and the cell wall.

Original languageEnglish
Pages (from-to)1186-1202
Number of pages17
JournalMolecular Microbiology
Volume77
Issue number5
DOIs
Publication statusPublished - szept. 2010

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Schizosaccharomyces
Cell Wall
Phosphotransferases
Cell Membrane
Mitogen-Activated Protein Kinases
Null Lymphocytes
Intracellular Membranes
Plasma Cells
Cytoskeleton
Morphogenesis
Catalytic Domain
Hypersensitivity
Adenosine Triphosphate
Alleles
Phenotype
Membranes

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Kin1 is a plasma membrane-associated kinase that regulates the cell surface in fission yeast. / Cadou, Angela; Couturier, Anne; Le Goff, Cathy; Soto, Teresa; Miklós, I.; Sipiczki, M.; Xie, Linfeng; Paulson, James R.; Cansado, Jose; Le Goff, Xavier.

In: Molecular Microbiology, Vol. 77, No. 5, 09.2010, p. 1186-1202.

Research output: Article

Cadou, A, Couturier, A, Le Goff, C, Soto, T, Miklós, I, Sipiczki, M, Xie, L, Paulson, JR, Cansado, J & Le Goff, X 2010, 'Kin1 is a plasma membrane-associated kinase that regulates the cell surface in fission yeast', Molecular Microbiology, vol. 77, no. 5, pp. 1186-1202. https://doi.org/10.1111/j.1365-2958.2010.07281.x
Cadou, Angela ; Couturier, Anne ; Le Goff, Cathy ; Soto, Teresa ; Miklós, I. ; Sipiczki, M. ; Xie, Linfeng ; Paulson, James R. ; Cansado, Jose ; Le Goff, Xavier. / Kin1 is a plasma membrane-associated kinase that regulates the cell surface in fission yeast. In: Molecular Microbiology. 2010 ; Vol. 77, No. 5. pp. 1186-1202.
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