The first isolation of reovirus type 1 from lambs is reported. The virus was isolated from the nasal and rectal swabs of lambs with respiratory and enteric disease. In the course of the epidemic two phases were observed, the first characterized with relatively mild clinical symptoms and followed by a secondary bacterial complication. The isolated virus is most likely to play a role in inducing the disease. The virus was isolated on foetal lamb kidney cells by inoculating both outgrown cell monolayers and cell suspensions. The latter method was more successful. The isolate was characterized by its cytopathic effect, morphology, resistance to heat, chloroform, sodium deoxycholate, saponin and trypsin and by its heat stability in the presence of divalent cations. Serologically it proved to belong to reovirus type 1.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology