In May 2008 a mass mortality event of brown bullheads (Ameiurus nebulosus) has been reported in a South-Hungarian reservoir near Szeged. Gross pathological, histopathological, virological and molecular biological examination of the fish samples revealed ranavirus infection. On the skin all over the body of the fish, on the gills and in the internal organs several haemorrhages have been detected. Virus isolation on EPC (Epithelioma Papulosum Cyprini) and BF-2 (Bluegill fibroblast) cell lines was performed and as a sign of virus replication rounding of the infected cells and cytoplasmic inclusion bodies could be observed by haematoxylin-eosin staining. Polymerase chain reactions (PCR) were carried out from the internal organ samples of the brown bullheads and EPC cell culture supernatant, targeting a portion of the iridoviral major capsid, DNA-polymerase and neurofilament triplet H1-like protein (NF-H1) coding genes. Sequence analysis of the PCR products and restriction endonuclease analysis of the NF-H1 gen PCR product revealed ranavirus infection most similar to the European sheatf ish and catfish viruses. In the study the authors describe the first detection of ranavirus in connection with the haemorrhagic syndrome of brown bullheads causing high mortality.
|Number of pages||6|
|Journal||Magyar Allatorvosok Lapja|
|Publication status||Published - dec. 2013|
ASJC Scopus subject areas